棉花TRY基因的电子克隆

[目的]克隆棉花TRIPTYCHON(TRY)基因.[方法]利用电子克隆和RT-PCR相结合的方法,克隆棉花TRY基因,并利用生物信息学分析软件,分析棉花TRY蛋白的生物信息.[结果]从徐州142无绒无絮突变体中,克隆到棉花TRY基因,其ORF(Open reading frame )全长270 bp,编码89个氨基酸.棉花TRY蛋白属于不稳定、疏水性蛋白,不存在信号肽及糖基化位点,没有跨膜结构,定位于细胞核,三级结构不存在卷曲螺旋结构.[结论]在棉花中利用电子克隆技术克隆TRY基因是可行的,同时研究结果为进一步研究棉花TRY基因的功能和棉纤维发育调控机制提供理论依据.

In Silico Cloning of Triptychon in Cotton

【Objective】Cloning TRIPTYCHON(TRY)in cotton.【Method】In silico cloning and RT-PCR techniques were combined to isolate a TRIPTYCHON(TRY) gene in cotton.The character of TRY protein were analyzed by a series of bioinformatics software.【Result】 TRIPTYCHON(TRY) gene was cloned in Xuzhou 142 lintfree mutants.Open reading frame(ORF) of TRY was 270bp in length,encoding 89 amino acids.TRY protein have no signal peptide,and no O-glycosylation site,no transmembrane domains,and no coiled coil structure;it is an unstable and hydrophobic protein,and located in the nucleus.【Conclusion】It is feasible to use in silico cloning techology to isolate TRY in cotton,and this research offered theoretical basis for the further research on the function of cotton TRY gene and regulatory mechanism for cotton fiber development.