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| 农杆菌介导法转化大豆体细胞胚获得转基因植株 | |
| 引用本文： | 王晓春,王 罡,王 萍,季 静,刘尚前.农杆菌介导法转化大豆体细胞胚获得转基因植株[J].中国农学通报,2006,22(4):40-40. |
| 作者姓名： | 王晓春 王 罡 王 萍 季 静 刘尚前 |
| 作者单位： | 1. 天津大学农业与生物工程学院,天津,300072;河北北方学院南校区农业科学系,河北,宣化,075131 2. 天津大学农业与生物工程学院,天津,300072 3. 淮海工学院海洋学院,连云港,222005 4. 河北北方学院南校区农业科学系,河北,宣化,075131 |
| 基金项目： | 国家科技部“国家植物转基因技术研究开发与中试基地建设”专项课题(J99-B-001)资助。 |
| 摘 要： | 以六个品种大豆体细胞胚为受体，以Bt基因为目的基因，应用根癌农杆菌介导法对大豆进行了遗传转化，经卡那霉素选择、诱导体细胞胚萌发及壮苗培养，获得了完整的抗性再生苗，经PCR、PCR-Southern和点杂交分析鉴定，初步证明外源基因Bt已整合到大豆的基因组中。 |
| 关 键 词： | 磁场 磁场 水稻 种子 NaCl胁迫溶液 种子萌发 |
| 收稿时间： | 2005-12-16 |
| 修稿时间： | 2005-12-162005-12-23 |
Genetic Transformation of somatic embryos viaAgrobacterium tumefaciens in Soybean |
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| Wang Xiaochun,Wang Gang,Wang Ping,Ji Jing,Liu Shangqian.Genetic Transformation of somatic embryos viaAgrobacterium tumefaciens in Soybean[J].Chinese Agricultural Science Bulletin,2006,22(4):40-40. | |
| Authors: | Wang Xiaochun Wang Gang Wang Ping Ji Jing Liu Shangqian |
| Institution: | 1Tian Jin University of Agricultural and Biological engineering Tian jin 300072; 2Department of agriculture sciences of Hebei North University, Xuan Hua 075131; 3Huahai Institute of Technology, Marine School,Lianyungang 222005 |
| Abstract: | Somatic embryos of 6 different soybean were transformed by Agrobacterium tumefaciens , The resistant plants to kanamycin were obtained. 5 positive plants were obtained among them by PCR , These positive plantlets were further analyzed by dot blotting and PCR-Southern blotting. These results demonstrated that the target gene had been transformed into the soybean genome. |
| Keywords: | Soybean Somatic embryos Bt gene Genetic transformation |
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