桔青霉素人工抗原合成及偶联比测定(摘要)(英文)

目的]建立一种快速准确鉴定和测定人工抗原偶联比的方法。方法]通过载体蛋白BSA合成桔青霉素人工抗原,用SDSPAGE电泳法、紫外吸收法、质谱法对其进行鉴定和测定。结果]经过3种方法的鉴定,桔青霉素人工抗原偶联成功,紫外吸收法测得其偶联比是8.4,质谱法测得其偶联比是6。结论]对3种供试方法比较发现,质谱法能够快速鉴定人工抗原而且能够准确测定人工抗原的偶联比。

Synthesis of Citrinin Artificial Antigen and Its Coupling Ratio Determination

Objective] This study was to establish a method for the rapid and accurate detection of the coupling ratio of artificial antigen. Method] Artificial synthetic antigen of citrinin （CIT） was first conjugated with vector protein bovine serum albumin （BSA） and then identified by SDS-PAGE electrophoresis,and its coupling ratio was measured by UV absorption and mass spectrometry. Result] The identification result showed that artificial antigen of CIT was successfully coupled,and the coupling ratio was 8.4 by UV absorption while 6.0 by mass spectrometry. Conclusion] The comparison experiment showed that mass spectrometry could rapidly identify the artificial antigen and accurately detect its coupling ratio. This study provided basis for the preparation of CIT antigen as well as the establishment of an for enzyme-linked immunosorbent assay （ELISA） for citrinin determination.