| 苹果花药培养植株倍性及其纯合基因型的鉴定 |
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| 引用本文： | 张利义,杨振英,丛佩华,张开春,刘建利,薛光荣.苹果花药培养植株倍性及其纯合基因型的鉴定[J].园艺学报,2007,34(2):481-484. |
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| 作者姓名： | 张利义 杨振英 丛佩华 张开春 刘建利 薛光荣 |
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| 作者单位： | (中国农业科学院果树研究所, 辽宁兴城125100; 北京市农林科学院林业果树研究所, 北京100093; 西北第二民族学院生命科学系, 银川750021) |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 用倍性分析仪对3株苹果花药培养植株进行了倍性鉴定, 并用AS2PCR分子标记分析其是否为纯合体。流式细胞法测定其倍性分别为二倍体、三倍体和四倍体; AS2PCR分析证明它们为纯合基因型。可认为花药培养植株的多倍化现象可能是在组织培养条件下, 由起源于单倍体的细胞发生自然加倍所形成的结果。
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| 关 键 词： | 苹果 花药培养 AS-PCR 倍性 纯合基因型 |
| 文章编号： | 0513-353X（2007）02-0481-04 |
| 收稿时间： | 2006-07-21 |
| 修稿时间： | 2006-07-212006-12-29 |
Analysis of Ploidy and Homozygous Genotype of Apple Plants Obtained by Anther Culture |
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| ZHANG Li-yi,YANG Zhen-ying,CONG Pei-hua,ZHANG Kai-chun,LIU Jian-li,XUE Guang-rong.Analysis of Ploidy and Homozygous Genotype of Apple Plants Obtained by Anther Culture[J].Acta Horticulturae Sinica,2007,34(2):481-484. |
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| Authors: | ZHANG Li-yi YANG Zhen-ying CONG Pei-hua ZHANG Kai-chun LIU Jian-li XUE Guang-rong |
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| Institution: | (;1.Institute of Pom ology, Chinese Academ y of Agricultural Sciences, Xingcheng, L iaoning 125100, China;2.Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, B eijing 100093, China;3.Departm ent of Life Sciences, the Second Northwest University forM inorities, Yinchuan 750021, China) |
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| Abstract: | In this paper,the ploidy and homozygous genotype of three apple plants obtained by anther culture were analyzed by using methods of the flow cytometric analysis and AS-PCR marker,in order to confirm their haploid origin.The results have proved that they all have a true homozygous status by AS-PCR analysis although they are now diploid,triploid and tetraploid respectively by Ploidy Analyser,which is possibly regarded as a result of spontaneous polyploidisation from haploid cell during tissue culture. |
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| Keywords: | AS-PCR |
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