| 玉米大斑病菌完整染色体DNA的制备方法 |
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| 引用本文： | 刘振盼,王艳辉,董金皋. 玉米大斑病菌完整染色体DNA的制备方法[J]. 华北农学报, 2007, 22(6): 176-178 |
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| 作者姓名： | 刘振盼 王艳辉 董金皋 |
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| 作者单位： | 河北农业大学,真菌毒素实验室,河北,保定,071001;河北农业大学,真菌毒素实验室,河北,保定,071001;河北农业大学,真菌毒素实验室,河北,保定,071001 |
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| 基金项目： | 河北省自然科学基金项目资助(303208) |
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| 摘 要： | 为建立一套全面的制备玉米大斑病菌完整染色体的制备体系,以玉米大斑病菌菌株F124为供试菌株,比较了冷冻液氮研磨法、直接包埋法、原生质体包埋法制备玉米大斑病菌完整染色体的效果。结果发现:使用直接包埋法不能从玉米大斑病菌中提取染色体;冷冻液氮研磨法得到的染色体是不完整的;原生质体法提取出了完整的染色体,是最适合制备完整染色体的方法。并且用原生质体法提取该菌株的染色体脉冲场电泳技术初步分离,首次得到了2条大于2200 kb的染色体条带。
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| 关 键 词： | 玉米大斑病菌 完整染色体DNA 电泳核型 |
| 文章编号： | 1000-7091（2007）06-0176-03 |
| 收稿时间： | 2007-10-23 |
| 修稿时间： | 2007-10-23 |
Study on Preparation Methods of Exserohilum turcicum for Intact Chromosome DNA |
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| LIU Zhen-pan,WANG Yan-hui,DONG Jin-gao. Study on Preparation Methods of Exserohilum turcicum for Intact Chromosome DNA[J]. Acta Agriculturae Boreali-Sinica, 2007, 22(6): 176-178 |
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| Authors: | LIU Zhen-pan WANG Yan-hui DONG Jin-gao |
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| Abstract: | Several preparation methods for Exserohilum turcicum intact chromosome DNA were comparatively studied,in order to establish an allsided system to analysis the molecular karyotype of this fungi.The results showed that chromosome DNA of Exserohilum turciczhubum were only obtained by the agarose-embedding protoplasts method,other methods gave incompetent results.Moreover,two chromosomal bands were separated by PFGE,both were more than 2 200 kb. |
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| Keywords: | Exserohilum turcicum Intact Chromosome DNA Electrophoretic karyotype |
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