药物对柔嫩艾美尔球虫内生阶段超微结构影响的研究

本文对柔嫩艾美尔球虫(Eimeria tenella)在鸡体内正常发育的超微结构和分别用三种抗球虫药物处理情况下超微结构的变化进行了观察和比较研究,注意到125ppm尼卡巴嗪(Nicarbazin)能引起裂殖生殖阶段的滋养体或初生裂殖体及裂殖子的线粒体、内质网等膜结构系统和核的异常变化;168ppm磺胺喹恶啉(SQ)对滋养体、初生裂殖体及裂殖子、大配子和卵囊壁的形成均有影响,对裂殖生殖阶段的影响主要表现为核膜的增生复制等一系列核的异常变化;对配子生殖阶段的影响表现为Ⅰ型和Ⅱ型卵囊壁形成颗粒(WFB_1和WFB_2)的异常变化;80ppm盐霉素(Salinomycin)对上皮内淋巴细胞(IEL)中的子孢子及粘膜中成熟裂殖子的影响表现为表皮的分离或破裂及胞浆内微线粒(MN)和多糖颗粒(PG)等的轮廓模糊。由药物引起的其他一些超微结构的异常变化也作了观察和描述。

THE EFFECT OF DRUGS ON THE UITRASTRUCTURE OF EIMERIA TENELLA IN ENDOGENOUS DEVELOPMENTAL STAGES

The ularastructural changes at merogeny and gametogony stages of Eimeria tenella were observed carefully in the cecal mucosa of chickens fed three different anticoccidial drugs and had been compared with the controls. Nicarbazin at 125 ppm, sulpbaquinoxaline ( SQ ) at 168 ppm and salinomycin at 80 ppm caused considerable morphological abnormalities at the parasites in the merogony. And also SQ could produce mor phological abnormalities at the wall-forming bodies of type I ( WFBI ) and type II ( WFB II ) in the gametogony. The membranous abnormality at the mitochondrion, endoplasmic reticulum, etc, and abnormal changes at the nucleus of trophozoite, young schizont and merozoite were seen in Nicarbazin treatment group. A series of changes in merogony C trophozoite, young Schizont and merozoite ) had taken place in SQ treatment group, mainly expressing by nucleus abnormalities, such as the duplicating at the nucleus membrane. The effect of salinomycin on sporozoites in the intraepithelial lymphcyte and on matural merozo-ites in the mucosa were expressed by the separation or destruction of pellicle and the indistinct outlines of micronemes , polysaccharide granule, etc. in the plasma. Other abnormalities resulting from drug treatment are also reported.