不同光谱能量分布对菊花试管苗增殖及生根的影响

采用发光二极管（LED）调制光质和光量，研究光谱能量分布对菊花离体培养增殖和生根阶段的影响，以荧光灯为对照。研究结果表明：红光有助于增加株高、节间长，且有利于生根试管苗可溶性糖、淀粉和碳水化合物的合成；蓝光显著提高丛生苗的叶绿素b、叶绿素总量及类胡萝卜素含量和生根试管苗游离氨基酸含量；而红蓝黄复合光不仅有利于丛生苗分化和增殖，也利于促生根组培苗色素形成、生长发育及根系活力。与荧光灯相比，红蓝黄复合光质LED具有明显优势，有利于提高增殖系数，培育壮苗和降低能耗成本。

Effects of Light Spectral Energy Distribution on Multiplication and Rooting of Chrysanthemum Plantlets in Vitro

The effects of different light spectral energy distribution of light-emitting diode（LED）on multiplication and rooting of chrysanthemum plantlets in vitro were studied. Fluorescent light was used as the control. The results showed that，red LED was in favour of enhancing plant height and internode length，and synthesizing soluble sugar，starch and carbohydrate；The content of chlorophyll b，chlorophyll a + b and carotenoid of shoots including content of amino acid of plantlets in vitro were significantly increased by blue LED；Complex of red，blue and yellow LED was not only suitable for differentiation and proliferation of shoots in vitro，but for pigment synthesis，growth and root vigor of plantlets in vitro. The results indicated that，as compared with fluorescent light，application of complex of red + blue + yellow LED was propitious to improve propagation coefficient，breed vigorous plantlets and decrease energy consumption.