番茄SlIAA14基因启动子克隆及分析

为了深入研究SlIAA14基因的表达模式及其调控番茄根系发育的分子机理，利用染色体步移技术从番茄品种‘Ailsa Craig’中克隆到该基因上游2 197 bp的启动子片段。生物信息学分析表明，该启动子片段中含有多个对脱落酸、赤霉素、细胞分裂素等激素响应的顺式作用元件，以及对干旱和伤害等逆境胁迫响应的顺式作用元件。利用农杆菌介导的遗传转化方法获得SlIAA14：：GUS + YFP融合基因的转基因番茄植株，转基因植株根系YFP和GUS检测结果显示，该启动子片段在番茄根尖和侧根原基有较高的表达活性，表明SlIAA14基因可能调控番茄根系的伸长和侧根发育的起始。

Cloning and Analysis of the Promoter of SlIAA14 in Tomato

Promoter analysis of SlIAA14 was carried out，to further study the expression profile of SlIAA14 and its regulatory mechanism in tomato root development. A 2 197 bp promoter fragment was cloned from tomato cultivar Ailsa Craig，using PCR-based genomic DNA walking. Several putative cis-elements responding to phytohormones（ABA，GA and CTK）and stress（dehydration and wounding）were predicted using online promoter analysis software. This promoter fragment was employed to drive YFP-GUS reporter genes，and was introduced into tomato using Agrobacterium-mediated transformation. YFP and GUS activity was detected in tomato root tip and lateral root primordium，suggesting SlIAA14 gene might participate in the root elongation and lateral root initiation of tomato.