Serum protein pattern in ewe with pregnancy toxemia |
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Authors: | Gul Fatma Yarim Gulay Ciftci |
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Institution: | (1) Department of Biochemistry, Faculty of Veterinary Medicine, University of Ondokuz Mayis, 55139 Samsun, Turkey |
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Abstract: | Pregnancy toxemia is a metabolic disease of pregnant ewes which causes significant economic losses in sheep industry. The
pathophysiology and metabolic changes of this disorder remain poorly understood. We conducted this study to describe the serum
protein pattern associated with the pregnancy toxemia in ewes. In this study, the electrophoretic pattern of serum proteins
of 15 ewes with naturally occuring pregnancy toxemia and 12 ewes with uncomplicated pregnant were investigated by Sodium Dodecyl
Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Serum protein patterns were mainly characterized by four bands and
located in the 76 kDa, 66 kDa, 55 kDa and 29 kDa both diseased and control groups. The percent of the 66 kDa, 55 kDa and 29 kDa
proteins were decreased (P < 0.001 for 66 kDa; P < 0.01 for 55 kDa and P < 0.05 for 29 kDa) while 76 kDa (P < 0.05) protein
was significantly increased (P < 0.001) in ewes with pregnancy toxemia relative to controls. Positive correlations were found
between activities of liver enzymes and percentage of the distribution in 76 kDa, 55 kDa proteins. In contrast, there was
a negative correlation between the 66 kDa protein and liver enzymes. In conclusion, the results of this study demonstrate
that the percentages of the 76 kDa, 66 kDa, 55 kDa and 29 kDa proteins are significantly altered in ewes with pregnancy toxemia.
However, further studies are needed to explore the potential role of these alterations in the pathophysiology in ewe with
pregnancy toxemia. |
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Keywords: | Ewe Pregnancy toxemia Protein pattern Serum |
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