| 红果冬青快繁技术的研究 |
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| 引用本文： | 张晓申,邢廷茂,王慧瑜,史喜兵,李晓青,赵海红.红果冬青快繁技术的研究[J].山东农业科学,2012,44(2):41-43. |
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| 作者姓名： | 张晓申 邢廷茂 王慧瑜 史喜兵 李晓青 赵海红 |
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| 作者单位： | 郑州市农林科学研究所,河南郑州,450005 |
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| 摘 要： | 以红果冬青当年生嫩枝茎段为外植体进行组织快繁试验。结果表明∶红果冬青的最佳诱导培养基为MS+0.2 mg/L NAA+0.9 mg/L BA,增殖培养基为MS+1.5 mg/L BA+0.5 mg/L IBA,生根培养基为1/2MS+1.0 mg/L IBA+0.3 mg/L NAA,生根率达到97.5%。经生根的试管苗移栽于蛭石(V)∶珍珠岩(V)∶泥炭(V)=2∶2∶1混合基质,控制温度20～30℃,相对湿度85%～90%,保湿20～25 d,其间适当遮阴,成活率可达91%。
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| 关 键 词： | 红果冬青 组织培养 快速繁殖 |
Tissue Culture and Rapid Propagation of Ilex purpure Hassk |
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| ZHANG Xiao-shen
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XING Ting-mao
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WANG Hui-yu
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SHI Xi-bing
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LI Xiao-qing
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ZHAO Hai-hong.Tissue Culture and Rapid Propagation of Ilex purpure Hassk[J].Shandong Agricultural Sciences,2012,44(2):41-43. |
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| Authors: | ZHANG Xiao-shen XING Ting-mao WANG Hui-yu SHI Xi-bing LI Xiao-qing ZHAO Hai-hong |
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| Institution: | (Zhengzhou Institute of Agricultural and Forestry Sciences,Zhengzhou 450005,China) |
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| Abstract: | The segments of shoots with buds were used as explants for tissue culture and rapid propagation of Ilex purpure Hassk.The results showed that the optimal induction medium was MS + 0.9 mg/L BA +0.2 mg/L NAA;the optimal medium for rapid propagation was MS+1.5 mg/L BA+0.5mg/L IBA;the optimal rooting medium was 1/2MS+1.0 mg/L IBA+0.3 mg/L NAA and the rooting rate was 97.5%.The survival percentage of plantlets could be up to 91% through transplanted in the substrate containing vermiculate,pearlite and peat(2∶2∶1,v/v) and cultured at 20～30℃ with the relative humidity of 85%～90% and appropiate shading for 20～25 days. |
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| Keywords: | Ilex purpure Hassk Tissue culture Rapid propagation |
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