| 一种鸡腿菇病害病原菌的分离及鉴定 |
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| 引用本文： | 兰玉菲,安秀荣,王庆武,常文程,唐丽娜.一种鸡腿菇病害病原菌的分离及鉴定[J].山东农业科学,2012,44(3):84-87. |
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| 作者姓名： | 兰玉菲 安秀荣 王庆武 常文程 唐丽娜 |
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| 作者单位： | 1. 泰安市农业科学研究院食用菌研究所,山东泰安,271000
2. 山东农业大学植物保护学院植物病毒研究室,山东泰安,271018 |
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| 基金项目： | 国家现代农业(食用菌)产业体系建设专项(CARS—24);山东省中青年科学家科研专项基金(BS2011NY021);泰安市科技发展计划项目(20113008) |
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| 摘 要： | 从莒县鸡腿菇种植区采集到表现鸡爪症状的样品,分离得到病原菌分离物RZ,采用改良的CTAB法提取其基因组总DNA,利用真菌通用引物ITS1/ITS4扩增其rDNA ITS区域序列,扩增产物纯化克隆后转化大肠杆菌DH5α,挑选阳性克隆进行测序。将获得的序列进行同源性比对和构建系统进化树,发现RZ分离物与GenBank中Bionectria ochroleuca 2个分离物聚为一簇,ITS的核苷酸一致率均为99.17%,从分子水平上证明该分离物为淡色生赤壳菌(B.ochroleuca)。
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| 关 键 词： | 鸡腿菇 病害 转录间隔区 分子鉴定 |
Isolation and Identification of One Kind of Pathogen from Coprinus comatus |
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| LAN Yu-fei
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AN Xiu-rong
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WANG Qing-wu
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CHANG Wen-cheng
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TANG Li-na.Isolation and Identification of One Kind of Pathogen from Coprinus comatus[J].Shandong Agricultural Sciences,2012,44(3):84-87. |
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| Authors: | LAN Yu-fei AN Xiu-rong WANG Qing-wu CHANG Wen-cheng TANG Li-na |
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| Institution: | 1(1.Edible Fungi Research Institute,Taian Academy of Agricultural Sciences,Taian 271000,China; 2.College of Plant Protection,Shandong Agricultural University,Taian 271018,China) |
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| Abstract: | The Coprinus comatus samples with the symptom of chicken claw were collected from Juxian areas,and a fungi strain named RZ was isolated.Its genome total DNA was obtained by improved CTAB method.The internal transcribed spacers(ITS) were amplified by PCR using fungi universal primers ITS1 and ITS4,and the amplified products were transformed into Escherichia coli DH5α after purified.Then the positive clones were screened and sequenced.The homology analysis showed that RZ was clustered with two Bionectria ochroleuca isolates in GenBank and shared 99.17% of nucleotide identity in ITS,which confirmed it to be Bionectria ochroleuca. |
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| Keywords: | Coprinus comatus Pathogen Internal transcribed spacers(ITS) Molecular identification |
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