Insulin-like growth factor I in sera, ovarian follicles and follicular fluid of cows with spontaneous or induced cystic ovarian disease |
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Authors: | Ortega Hugo H Palomar Martin M Acosta Juan C Salvetti Natalia R Dallard Bibiana E Lorente Juan A Barbeito Claudio G Gimeno Eduardo J |
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Affiliation: | Department of Anatomy and Histology, Faculty of Veterinary Sciences, National University of Litoral, R.P. Kreder 2805, CP 3080, Esperanza, Santa Fe, Argentina. hhortega@fcv.unl.edu.ar |
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Abstract: | The objective of this research was to determine changes in IGF-I levels in serum and follicular fluid, and immunoreactivity of the follicle wall of cows with spontaneous (slaughter specimens) or ACTH-induced follicular cysts, and to compare results to normal cycling (control) cows after selection of the ovulatory follicle. Concentrations of IGF-I in serum did not differ between control and cystic animals (p=0.76). Fluid from the ovulatory follicle in control cows had 41% higher concentrations of IGF-I than that from cystic follicles collected at slaughter (spontaneous cysts; p<0.05) and 70% higher than that in induced follicular cysts (p<0.05). An intense positive immunostaining with anti-IGF-I was observed in granulosa cells (p<0.05) and in the theca interna (p<0.05) of secondary and tertiary follicles in all three groups of animals, but staining was less intense in cystic (p<0.05) and atretic follicles (p<0.05). This study provides evidence to suggest that cystic ovarian disease in cattle is associated with decreased concentrations of IGF-I in follicular fluid, but not in serum, and decreased production of IGF-I in the follicular wall. These data support the notion that IGF-I plays a role in the regulation of folliculogenesis, and may participate in the pathogenesis of cystic ovarian disease in cattle. |
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Keywords: | IGF-I Growth factors Cow Cystic ovarian disease Ovary Anestrous |
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