镰刀菌rDNA ITS区 RFLPs分析

本实验采用PCR-RFLP技术分析了镰刀菌菌种间的多态性。运用SDS碱裂解法抽提了采自于山西省不同地区的11株镰刀菌（Fusarium）基因组DNA，并用一对特异性引物对其rDNA ITS区段进行扩增，选用4种限制性内切酶（TaqⅠ AluⅠ HaeⅢ EcoRⅠ）酶切PCR扩增产物，共得到16条多态性片段，其中特异性条带8条，分子量大约在70-900bp之间。利用NTSYS-PC(2.1)软件包分析各菌株间的DNA限制性片段多态性，总共可以分为５大组，与传统分类一致，说明该方法可以用于镰刀菌菌株间的多态性分析。

RFLPs Analysis In The Region of rDNA ITS of Fusarium

This experiment applies technique to analyze the polymorphism of the Fusarium strains. Using SDS alkaline lysis method extract the genomic DNA of eleven Fusarium which from the different part of Shanxi province. Amplifying the rDNA ITS region of these eleven Fusarium, which used one pair of differential primer, and betaken four restriction enzymes (TaqI, AluI, HaeⅢ, EcoRI) to digest the PCR amplification .Totally it gains sixteen polymorphic fragment which include eight special band, it’s molecular weight is 70-900bp. Finally adopted NTSYS-PC(2.1) package analyze the DNA restriction fragment polymorphism among every strains, which is classified five big groups and is coincident with the traditional classification. The result shows that this method can be used to analyze polymorphism among the Fusarium strains.