Effect of modified oligopeptides from the beetle Allomyrina dichotoma on Escherichia coli infection in mice |
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Authors: | Yamada Manabu Nakamura Kikuyasu Saido-Sakanaka Hisako Asaoka Ai Yamakawa Minoru Sameshima Toshiya Motobu Maki Hirota Yoshikazu |
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Affiliation: | National Institute of Animal Health, Tsukuba, Ibaraki, Japan. |
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Abstract: | The novel peptides based on a putative active site of defensin, an anti-bacterial peptide from the beetle Allomyrina dichotoma, were synthesized. These synthetic oligopeptides exhibited strong anti-bacterial activity in vitro, even against antibiotic-resistant pathogenic bacteria. Then, anti-bacterial activity of two newly synthesized peptides, RLYLRIGRR-NH(2) (peptide A) and RLRLRIGRR-NH(2) (peptide B) was also examined by macroscopic and histopathologic assessment during the course of infection in mice inoculated with antibiotic-resistant pathogenic Escherichia coli (E. coli) in vivo. Peptide B decreased the mortality of mice inoculated with antibiotic-resistant pathogenic E. coli. The results of macroscopic and histopathologic examinations revealed that peptide B could protect the mice from infection. In contrast, peptide A failed to protect mice from infection with antibiotic-resistant pathogenic E. coli. Also, modified peptides A and B produced no toxicity or side effects in mice. These results suggest that peptide B is useful for developing novel antibiotics against antibiotic-resistant pathogenic bacteria. |
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