镉对体外培养大鼠肝细胞自噬的影响

选用体外培养原代大鼠肝细胞为模型，醋酸镉处理之后，用Westernblot和免疫荧光法检测了自噬标记分子LC3的表达水平，在此基础上，利用自噬激活剂（雷帕霉素）诱导自噬水平的升高，用MTT法分析了镉对细胞存活率的影响。结果显示，原代大鼠肝细胞在体外培养过程中自噬水平具有缓慢上升的趋势，而镉处理延缓了这种趋势，降低了自噬的水平和细胞存活率；利用激活剂增强自噬后不能减弱镉的损伤，表明镉有可能通过抑制自噬的保护作用，造成对肝细胞的损伤。

Effects of cadmium on autophagy in primary rat hepatocytes cultured in vitro

In the present research,cadmium treated primary rat hepatocytes cultrued in vitro as the model. As a marker of autophagy,microtubule-associated proteinl light chain 3 （LC3） was exam- ined by western blot and immunofluorescence staining. Effect of cadmium on cell viability was ex- amined by MTT method, after rapamycin induce authophagy. The results showed that autophagy slowly increased during in vitro culture of primary rat hepatocytes, and cadmium treatment inhibi- ted this process by decreased autophagy and cell viability. However,activation of autophagy by ra- pamycin did not repair the damage of cadmium. These results indicate that cadmium may inhibit the protection of autophagy to damage the hepatocytes.