| 应用Mab-basedAC—ELISA法对鸡IBDV分离株的抗原特性分析 |
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| 引用本文： | 张艳英,高桂生,贾青辉,高光平,李蕴玉,张香斋,孙继国,张曼夫,常火炬,李佩国.应用Mab-basedAC—ELISA法对鸡IBDV分离株的抗原特性分析[J].兽医大学学报,2013(12):1828-1831,1837. |
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| 作者姓名： | 张艳英 高桂生 贾青辉 高光平 李蕴玉 张香斋 孙继国 张曼夫 常火炬 李佩国 |
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| 作者单位： | [1]河北科技师范学院,河北预防兽医学重点实验室,河北昌黎066600 [2]中国农业大学,北京100193 [3]河北农业大学,河北保定071001 [4]邯郸市丛台区动物卫生监督局,河北邯郸056004 |
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| 基金项目： | 基金项目：河北省自然科学基金资助项目（300115）;河北省科技攻关计划（12220408D）;国家自然科学基金资助项目（31072004）;河北科技师范学院科研创新团队（CXTD2012-01） |
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| 摘 要： | 为了解传染性法氏囊病病毒（IBDV）的抗原变异分子基础，采用Mab—basedAC—ELISA方法，利用11株单抗对2株河北IBDV分离株进行了抗原特性分析，并比较了超强毒株和弱毒株的VP2高变区序列的分子特征。结果显示：强弱毒株的抗原位点结合单抗的能力明显不同，除了Mab3、Mab9外，其余9个单抗的结合能力，超强毒株要高于弱毒株。超强毒株HeB-If缺少了Mab3结合位点，弱毒株HeB-bdjz缺少了Mab3、Mab6结合住点，这说明强弱毒株不仅在序列上有差异，而且在抗原位点的缺失上也存在差异。本试验为控制IBD和疫苗改进具有重要意义。
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| 关 键 词： | 鸡 IBDV Mab—based AC ELISA 抗原特性分析 |
Antigenic characteristic analysis of chicken IBDV isolates with Mab-based AC ELISA |
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| Authors: | ZHANG Yan-ying GAO Gui-sheng JIA Qing-hui GAO Guang-ping LI Yun-yu ZHANG Xiang- zhai SUN Ji-guo ZHANG Man-fu CHANG Huo-ju LI Pei-guo |
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| Institution: | 1. Key Laboratory of Preventive Veterinary Medicine of Hebei , Hebei Normal University of Science g〉 Technology, Changli, Hebei 066600, China 2. China Agricultural University, Beijing, 100094, China ; 3. Agri- cultural University of Hebei ,Baoding, Hebei 071001, China ; 4. Congtai Area Health Inspection of Handan , Handan , Hebei 056004, China) |
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| Abstract: | In order to understand molecular basis of antigenic variation on infectious bursal disease virus,antigenic characterstics of 2 IBDV isolates from Hebei province were analyzed to bind 11 Mabs with monoclonal antibody-base antigen capture ELISA（Mab-based AC-ELISA） in the test. And molecular characteristics of VP2 hypervariable regions were compared between very virulent isolates and attenuated isolates. The results showed that it is obviously distinct on ability of anti- gen site binding Mab. Apart from Mab3 and Mab9,in antigen capture ability of other 9 Mabs very virulent IBDV（vvIBDV）isolates are higher than that of attenuated isolates. Mab3 antigen-bind site was not detected in Hebei-lf isolates,while Mab3 and Mab6 site were both detected in HeB-bdjz i- solates. This suggested that it is different in the sequence and antigen site missing between very virulent IBDV and attenuated isolates. |
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| Keywords: | chicken IBDV Mab-based AC-ELISA antigenic characteristics analysis |
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