磺胺母核人工半抗原的合成与鉴定 |
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引用本文: | 杜玉玲,陆彦,祝文琪,高磊,岳城,吴国娟.磺胺母核人工半抗原的合成与鉴定[J].兽医大学学报,2013(12):1875-1878. |
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作者姓名: | 杜玉玲 陆彦 祝文琪 高磊 岳城 吴国娟 |
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作者单位: | [1]北京农学院动物科学技术学院,北京102206 [2]新疆农业大学动物医学学院,新疆乌鲁木齐830052 |
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基金项目: | 基金项目l国家自然科学基金(31172362,30972215);北京市属高校人才强教深化计划;北京市自然科学基金(5102014);北京市教委项目(KM201010020008) |
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摘 要: | 合成磺胺母核人工半抗原,并对其产物进行鉴定。采用4-氨基苯甲酸甲酯(PBPA)和对乙酰氨基苯磺酰氯(ASC)为原料,经过亲和取代,酯的水解反应合成磺胺药物共有的母核结构苯甲酸对氨基苯磺酰胺(SH),应用质谱法(ESI—MS)与核磁共振氢谱法(1H—NMR)鉴定SH。质谱结果显示M-]=291.03766,与理论值M]-292相符,表明SH合成成功;核磁共振氢谱(1H—NMR)的数据与SH化合物的结构相符。磺胺母核人工半抗原合成成功,为研制磺胺药多残留检测试剂盒和胶体金免疫层析试纸条奠定基础。
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关 键 词: | 磺胺母核 半抗原 合成 鉴定 |
The synthesis and identification of sulfanilamides artificial hapten |
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Authors: | DU Yu-ling LU Yan ZHU Wen-qi GAO Lei YUE Cheng WU Guo-juan |
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Institution: | 1. Department of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China ; 2. College of Veterinary Medicine ,Xinjiang University of Agriculture ,Urumqi 830052 ,China) |
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Abstract: | The sulfonamides artificial nucleus hapten was synthesized and identified. Using 4-amino- benzoate (PBPA) and acetylamino-benzenesulfonyl chloride(ASC) as raw material, after nueleo- philic substitution, through sulfa drugs mother nucleus structure, benzoic aminobenzene sulfona- mide(SH) was synthesized ester hydrolysis reaction. Mass spectrometry(ESI-MS) and proton nu- clear magnetic resonance spectroscopy(1 H-NMR) were applied for identification of SH. Mass spec- trometry results showed that the M-] = 291. 03766 is concordant with the theoretical value of the M]= 292,indicating the success of SH synthesis;NMR (1H-NMR) data match the structure of SH compound. The sulfonamides artificial nucleus hapten were synthesized successfully, which lay a foundation for the development of sulfa drugs multi-residue detection kit and colloidal gold immunochromatographic strip. |
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Keywords: | sulfonamides hapten synthesis identification |
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