白牦牛卵泡液蛋白质组2-DE图谱的构建

分别用10％、12％、14％的SD＆PAGE胶分离蛋白质，比较不同浓度胶对白牦牛卵泡液蛋白双向电泳分离效果的影响；分别用丙酮沉淀法、热的SDS法和直接溶解法制备样品，比较不同制备方法对白牦牛卵泡液蛋白双向电泳分离效果的影响。结果表明，不同浓度胶对蛋白分离效果影响较大；丙酮沉淀法、热的SDS法和直接溶解法获得的蛋白点数分别为364±36、290±19和374±30个。对于白牦牛卵泡液蛋白来说，将其直接溶解后，用12％的胶可以得到较理想的2～DE图谱。

The construction of 2-DE map of white yaks follicular fluid proteome

To study the effect of different concentration homogeneous gels of SDS-PAGE on white yaks follicular fluid proteins of separation in two-dimensional gel electrophoresis, the gel concen- trations of 10%,12% and 14% were applied to separate follicular fluid sample. To study the effect of different sample preparation methods on white yaks follicular fluid proteins of separation in two-dimensional gel electrophoresis, the acetone precipitation, hot SDS methods and direct solubili- zation were applied to prepare follicular fluid sample. The result showed that there was a great difference in proteins of separation in different concentrations gels. The total protein number of samples processed by acetone precipitation,hot SDS methods and direct solubilization was 364± 36,290±19 and 374±30 respectively. For the separation of white yak follicular fluid proteins in 2- DE,direct solubilization and 12.5% homogeneous gel can obtain perfect maps of 2-DE.