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Characterization of coagulase negative staphylococci from cases of subclinical mastitis in dairy cattle in Kampala,Uganda
Authors:Sandra Bj?rk  Renee B?ge  Benon M Kanyima  Susanne André  Maria G Nassuna-Musoke  David O Owiny  Ylva Persson
Institution:1.Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden;2.College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, Uganda;3.Department of Bacteriology, National Veterinary Institute (SVA), Uppsala, Sweden;4.Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute/Växa Sverige, Uppsala, Sweden
Abstract:

Background

Coagulase negative staphylococci (CNS) are the most common pathogens leading to subclinical mastitis (SCM) in dairy cattle in Uganda. Coagulase negative staphylococci can vary between bacterial species in how they cause disease. The aim of the study was to characterize CNS, from cows with SCM in Uganda, at the species level.

Findings

Quarter milk samples (n = 166) were collected from 78 animals with SCM. Bacteriological analyses were carried out at Makerere University, Kampala, Uganda and at the National Veterinary Institute (SVA), Uppsala, Sweden. The most common pathogens found in milk samples from cows with SCM were CNS (31.7%). Two species of CNS were found, S. epidermidis (85%) and S. haemolyticus (15%). Of the CNS isolates, 16/20 (80%) were positive for β-lactamase production (β+).

Conclusions

In milk samples from cows with SCM caused by CNS, S. epidermidis was most prevalent, followed by S. haemolyticus.
Keywords:CNS  Staphylococcus epidermidis  Staphylococcus haemolyticus  MALDI-TOF
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