普通小麦多酚氧化酶活性的QTL分析

多酚氧化酶（polyphenol oxidase, PPO）是引起面团（片）颜色褐变的主要原因。利用122对SSR引物、4对贮藏蛋白的STS引物和10对AFLP引物组合，分析了中优9507´CA9632的71个DH系的基因型，构建了由173个位点组成的遗传连锁图，在小麦21个连锁群上覆盖2 881 cM。将该群体种植于3种环境，采用复合区间作图法（CIM）进行了P

Mapping QTLs for Polyphenol Oxidase Activity in a DH Population from Common Wheat

The polyphenol oxidase (PPO) activity is a major factor to cause an undesirable brown discoloration of wheat-based end products during processing or storage.A double haploid (DH) population with 71 lines derived from Zhongyou 9507 with higher PPO activity and CA9632 with lower PPO activity, were sown at three locations with two replications.A total of 173 loci included SSR, STS of storage protein and AFLP markers were used to construct a linkage map, covering 2 881 cM of whole genome at 21 linkage groups.With the method of composite interval mapping (CIM), two putative major QTLs were detected on chromosome 2AL and 2DL, accounting for 37.9%-50.0% and 25%-29.1% of the phenotypic variance across three environments, respectively.The QTL on 2AL played a more significant role than the QTL on 2DL.Reduction of PPO activity through genetic improvement and molecular marker assisted selection was also discussed.