一株核桃树内生真菌的分离鉴定及活性研究

目的]从核桃树皮内分离筛选具抑菌活性的内生真菌菌株。方法]核桃树皮经自来水冲洗后置于培养基以产生真菌菌株,对获得的菌株进行形态学观察及抑菌活性测试。结果]从核桃树皮内分离到一株产广谱、高活性抑菌物质的内生真菌。经测定对细菌、植物病原真菌和皮肤致病真菌共24种病原微生物有不同程度的抑制作用。形态特征表明,该菌株与拟青霉属Paecilomyces Bainier中的淡紫拟青霉Paecilomyces lilacinus的特征基本一致,ITS序列分析显示本菌株与各淡紫拟青霉同源性都高于99%,因此将菌株JY-1024命名为淡紫拟青霉Paecilomyces lilacinusJY-1024。结论]该研究首次从核桃树皮内分离得到淡紫拟青霉并首次报道了其抑菌活性。

Isolation and Identification of an Endophytic Fungus Associated with Juglans regia L. and Its Bioactivity

Objective] This study was to screen out endophytic fungal strains from the bark of Juglans regia L. Method] The bark of J. regia was washed with running water and placed on medium to let the fungi grown; the fungal colonies were studied via morphological observation and anti-microbial activity. Result] An endophytic fungus showing broad-scope, highly active anti-microbial activity was screened out. This fungus showed anti-microbial activity seven Gram-positive and four Gram-negative bacterial indicators, six skin pathogenic fungi and seven pathogenic fungi to different extent. Morphological observation on the ITS sequence showed that this fungus is consistent with Paecilomyces lilacinus in genus Paecilomyces bainier(with homology of over 99%). Accordingly this fungus was designated as Paecilomyces lilacinus JY-1024. Conclusion] This study for the first time reported the isolation of P. lilacinus from J. regia and its in vitro antimicrobial activity to several pathogens.