海带多酚的提取和抑菌研究

[目的]对海带多酚的提取进行优化,并研究其分离纯化和抑菌作用。[方法]以新鲜海带为试验材料,采用以乙醇为溶剂反复冻融法提取海带多酚,液相色谱分离纯化海带多酚,并利用96孔板进行抑菌试验。[结果]海带多酚提取的最佳条件为:80%的乙醇浸提,60℃浸提3～5 h,提取效果最好。海带多酚的最大吸收在波长574 nm。高效液相色谱分析得到9种组分,经过抑菌试验获得5种抑菌组分。多酚对副溶血弧菌、大肠杆菌、藤黄叠球菌和金黄色葡萄球菌有一定的抑菌作用,对枯草芽孢杆菌无抑菌效应。[结论]试验为深入研究海带多酚提供了参考依据,同时也为海带的高值化利用及新的抗肿瘤药物开发提供一种新的思路。

Study on Kelp Polyphenols Extraction and Antibacterial Activity

[ Objective ] The aim was to optimize the extraction of kelp polyphenols, and study the separation and purification, baeteriostatic effects on kelp polyphenols component. [ Method] With fresh kelp as experimental material, using repeated freeze-thawing method to extract kelp polyphenols, and liquid chromatogram to separate and purify kelp polyphenols. Furthermore, 96 orifice plate was adopted to analyze anti- bacterial effects. I Result l The optimum extraction conditions of kelp polyphenols are： 80% ethanol extraction, 60 ℃ leaching 3 -5 h had better extraction effect. The maximum absorption of kelp polyphenols is at 574 nm. By high performance liquid chromatography （HPLC） anal- ysis obtain 9 components, 5 kinds of component had antibacterial function. Polyphenols had certain bacteriostatie action on the vice Hemolysis vibrio , Escherichia coli, Staphylococcus aureus and Gambogic fold coccus. Polyphenols had not bacteriostatic effect on Bacillus subtills. [Conclu- sion] The study provides a test basis for further research on kelp polyphenols and a new train of thought for kelp high threshold use and new antitumor drug development.