北五味子药材指纹图谱研究 |
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引用本文: | 王佩龙,刘爽,杨丽花,令狐浪,邹妍琳,廖志华,陈敏. 北五味子药材指纹图谱研究[J]. 安徽农业科学, 2013, 0(7): 2841-2843,2861 |
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作者姓名: | 王佩龙 刘爽 杨丽花 令狐浪 邹妍琳 廖志华 陈敏 |
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作者单位: | 西南大学药学院,发光与实时分析教育部重点实验室,重庆400715;西南大学生命科学学院,重庆,400715 |
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基金项目: | 科技部"重大新药创制"科技重大专项"创新药物质量控制研究平台",国家自然科学基金,西南大学"中央高校基本科研业务费专项资金" |
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摘 要: | [目的]建立北五味子药材的HPLC指纹图谱,为北五味子药材的质量控制提供保证。[方法]采用RP-HPLC分析,利用中药指纹图谱相似度评价系统(2004A版)对不同批次北五味子药材HPLC图谱进行分析比较。[结果]建立了北五味子药材的HPLC指纹图谱,以五味子醇甲、五味子甲素、五味子酚、五味子乙素为参照峰确立了北五味子药材指纹图谱中的35个共有峰。[结论]建立的指纹图谱重现性及稳定性均较好,可用于北五味子药材的质量管理规范。
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关 键 词: | 北五味子 HPLC 指纹图谱 质量控制 五味子醇甲 |
Study on HPLC Fingerprint of Herba Schisandra chinensis (Turcz.) Bail |
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Affiliation: | WANG Pei-long et al(Key Laboratory on Luminescence and Real-Time Analysis,School of Pharmaceutical Sciences,Southwest University,Chongqing 400715) |
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Abstract: | [Objective] To establish the chromatographic fingerprint for herba Schisandra chinensis(Turcz.)Bail by RP-HPLC,and provide a guaranteeing for quality control of S.chinensis.[Method] By using RP-HPLC analysis,the chromatograms of different batches of herba S.chinensis were compared by the software of similarity evaluation system for chromatographic fingerprint of TCM(Version 2004A).[Result] HPLC fingerprint of herba S.chinensis was established with schisandrin,schisanhenol,schizandrin a,schizandrin B as the reference compound.Eighteen mutual peaks were selected as the fingerprint peaks of S.chinensis.[Conclusion] The method can be provided as a scientific and effective way for quality control of S.chinensis. |
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Keywords: | Schisandra chinensis(Turcz.) Bail HPLC Fingerprint Quality control Schisandrin |
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