Induction of heat shock protein genes by chlorfenapyr in cultured cells of the cabbage armyworm,Mamestra brassicae |
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| Affiliation: | 1. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China;2. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, PR China;1. Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China;2. The Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, Sericultural Research Institute, Chinese Academy of Agricultural Science, Zhenjiang 212018, China;1. State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China;2. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China;3. School of Life Sciences, Peking University, Beijing 100871, China;4. Division of Plant Science and Technology, University of Missouri, Columbia, MO 65211, USA;1. Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Tai''an, Shandong 271018, PR China;2. College of Horticultural Science and Engineering, Shandong Agricultural University, Tai''an, Shandong 271018, PR China |
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| Abstract: | Effects of structurally different insecticides, permethrin, chlorfluazuron, chlorfenapyr, prothiofos, methomyl, and thiocyclam on expression of hsp90, hsp70, hsp20.7, and hsp19.7 were examined using cultured cells of the cabbage armyworm, Mamestra brassicae. Significant induction of hsp90, hsp70, hsp20.7, and hsp19.7 expression was observed in response to chlorfenapyr. No induction was observed for the remaining five insecticides. The chlorfenapyr-induced expression was time-dependent and concentration-dependent. A significant reduction in expression levels of hsp70, hsp20.7, and hsp19.7 was observed in the time course when the cells exposed to chlorfenapyr were transferred to chlorfenapyr-free medium. These results suggest that hsp70, hsp20.7, and hsp19.7 might be useful to assess cellular distress or injury by chlorfenapyr. |
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