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Effects of radiation on the fitness,sterility and arbovirus susceptibility of a Wolbachia-free Aedes albopictus strain for use in the sterile insect technique |
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| Authors: | Dong-jing Zhang Yan Sun Hanano Yamada Yu Wu Gang Wang Qing-deng Feng Dilinuer Paerhande Hamidou Maiga Jérémy Bouyer Jun Qian Zhong-dao Wu Xiao-ying Zheng |
| Affiliation: | 1. Chinese Atomic Energy Agency Center of Excellence on Nuclear Technology Applications for Insect Control, Key Laboratory of Tropical Disease Control of the Ministry of Education, Sun Yat-sen University, Guangzhou, China;2. Chinese Atomic Energy Agency Center of Excellence on Nuclear Technology Applications for Insect Control, Key Laboratory of Tropical Disease Control of the Ministry of Education, Sun Yat-sen University, Guangzhou, China SYSU Nuclear and Insect Biotechnology Co., Ltd, Dongguan, China International Atomic Energy Agency Collaborating Centre, Sun Yat-sen University, Guangzhou, China Guangdong Provincial Engineering Technology Research Center for Diseases-Vectors Control, Sun Yat-sen University, Guangzhou, China;3. Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna, Austria;4. Chinese Atomic Energy Agency Center of Excellence on Nuclear Technology Applications for Insect Control, Key Laboratory of Tropical Disease Control of the Ministry of Education, Sun Yat-sen University, Guangzhou, China International Atomic Energy Agency Collaborating Centre, Sun Yat-sen University, Guangzhou, China Guangdong Provincial Engineering Technology Research Center for Diseases-Vectors Control, Sun Yat-sen University, Guangzhou, China;5. Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, Vienna, Austria Institut de Recherche en Sciences de la Santé, Direction Régionale de l'Ouest, Bobo-Dioulasso, Burkina Faso |
| Abstract: | BACKGROUNDThe sterile insect technique (SIT) is a green and species-specific insect pest control technique that suppresses target populations by releasing factory-reared, radiosterilized males into the wild. Once released, it is important to be able to distinguish the released males from the wild males for monitoring purposes. Several methods to mark the sterile males exist. However, most have limitations due to monetary, process efficiency, or insect quality. Aedes albopictus is naturally infected with Wolbachia at a high prevalence, therefore the elimination of Wolbachia can serve as a biomarker to distinguish factory-reared male mosquitoes from wild conspecifics.RESULTSIn this study, a Wolbachia-free Ae. albopictus GT strain was developed and its fitness evaluated, which was found to be comparable to the wild GUA strain. In addition, GT male mosquitoes were irradiated at the adult stage and a dose of 20 Gy or more induced over 99% sterility. Moreover, a dose of 30 Gy (almost completely sterilizing male and female mosquitoes) had limited effects on the mating competitiveness of GT males and the vector competence of GT females, respectively. However, radiation reduced mosquito longevity, regardless of sex.CONCLUSIONOur results indicate that the Ae. albopictus GT strain can be distinguished from wild mosquitoes based on Wolbachia status and shows similar fitness, radio-sensitivity and arbovirus susceptibility to the GUA strain, indicating that it is feasible to use the GT strain to suppress Ae. albopictus populations for SIT programmes. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. |
| Keywords: | fitness adult irradiation arbovirus susceptibility mosquito control |