| 木薯叶过氧化物酶的分离纯化及其同工酶的研究 |
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| 引用本文： | 陈燕珍,谭涛,杨栋林,刘洁雯,李谋岚. 木薯叶过氧化物酶的分离纯化及其同工酶的研究[J]. 安徽农业科学, 2009, 37(36): 17872-17873 |
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| 作者姓名： | 陈燕珍 谭涛 杨栋林 刘洁雯 李谋岚 |
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| 作者单位： | 1. 广西师范大学生命科学学院,广西桂林,541004;广西环境工程与保护评价重点实验室,广西桂林,541004
2. 广西师范大学生命科学学院,广西桂林,541004 |
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| 基金项目： | 广西环境工程与保护评价重点实验室研究基金项目 |
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| 摘 要： | [目的]探讨将木薯叶作为过氧化物酶（POD）制备原料的可行性。[方法]木薯叶和甘蔗叶经提取液提取、盐析法沉淀,得POD粗酶液,分别用Sephadex G-75层析法和切向流超滤法纯化POD,用聚丙烯酰胺凝胶电泳（PAGE）法分离了POD同工酶。[结果]木薯叶和甘蔗叶POD的酶活分别为6.617×10~4和5.883×10~3U/g,前者远高于后者。SephadexG-75层析法分离POD粗酶液得到洗脱峰Ⅰ和Ⅱ,木薯叶和甘蔗叶都是峰Ⅰ有酶活,峰Ⅱ酶活很低甚至没有。POD粗酶液经切向流超滤后,木薯叶和甘蔗叶POD的回收率分别为91.825%和90.820%,RZ值分别为2.75和2.85。约在430nm处有最大吸收峰。木薯叶POD有3条与标样辣根POD相同的同工酶带。[结论]木薯叶POD含量高,提取纯化操作简便,有开发利用价值。
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| 关 键 词： | 木薯叶 甘蔗叶 过氧化物酶 超滤 电泳 |
Separation and Purification of Peroxidase from Cassava Leaves and Study on Its Isozyme |
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| CHEN Yan-zhen et al. Separation and Purification of Peroxidase from Cassava Leaves and Study on Its Isozyme[J]. Journal of Anhui Agricultural Sciences, 2009, 37(36): 17872-17873 |
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| Authors: | CHEN Yan-zhen et al |
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| Affiliation: | CHEN Yan-zhen et al(College of Life Science,Guangxi Normal Univeisity,Guilin,Guangxi 541004) |
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| Abstract: | [Objectives]To investigate the feasibility that cassava leaves are used as the raw materials of peroxidase(POD).[Methods]POD crude enzyme solutions were obtained by extracting cassava leaves and sugar cane leaves and,in turn,salting - out precipitation.Purification of POD was performed by using Sephadex G-75 gel chromatography and tangential flow ultrafiltration,respectively.POD isozymes were separated by using polyacrylamide gel electrophoresis(PAGE).[Results]The POD activities of cassava leaves and sugar ... |
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| Keywords: | Cassava leaves Sugercane leaves Peroxidase Ultrafiltration Electrophoresis |
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