箬叶提取物的制备及抗氧化活性研究

摘要：采用热水煮提、配比不同浓度的乙醇从箬叶中提取水溶性、醇溶性提取物，正交实验表明：水提箬叶工艺条件为箬叶、水配比15g：200ml，110℃，时间120min,测得的吸光度值高；醇提的工艺条件为时间90min、温度60℃、质量分数为90%乙醇时测得的吸光度值高。箬叶水提取物在浓度0.4～51.2mg/ml之间对超氧阴离子自由基清除能力呈增长趋势,当浓度为51.2mg/ml时,达到33.33%,而乙醇提取物对超氧阴离子的清除能力呈负增长趋势；水提取物对羟基自由基清除能力，在浓度0.4～51.2mg/ml之间呈增长趋势，当浓度为51.2mg/ml时,达到13.88%；而乙醇提取物呈负增长趋势，当提取物浓度超过6.4mg/ml时，呈负增长趋势显著增加，达-93.75%。总的来说，箬叶水提取物具有较强的抗氧化能力，有广泛的应用价值。

Study on Preparation and Antioxidant Activity of Extract from Indocalamus leaves

Abstract: By using the method of boiling-water extraction、ethanol extraction and orthogonal, the water-soluble and extracts ethanol-soluble extracts were obtained from Indocalamus leaves, and the results showed that when the water-soluble extraction condition was at the ratio of material 15g to liquid 200ml, extracting temperature 110℃ and extracting time 120min, the absorbance tested was highest. The ethanol-soluble extraction condition was extracting time 90min , extracting temperature 110℃,and quality score 90%, and the absorbance tested was highest. In the experiment of scavenging activity to O2-,the concentration of water-soluble extracts between 0.4～51.2mg/ml showed growing tendency, when the concentration was 51.2mg/ml,the scavenging rate was 33.33%. The ethanol-soluble extracts showed negative growing tendency. In the experiment of scavenging activity to •OH, the concentration of water-soluble extracts between 0.4～51.2mg/ml showed growing tendency, when the concentration was 51.2mg/ml,the scavenging rate was 13.88%. The ethanol-soluble extracts showed negative growing tendency. When the concentration exceed 6.4mg/ml, negative growing tendency was significant, even reached -93.75%. In conclusion, the water-soluble extracts of Indocalamus leaves had relatively strong antioxidant activity, so its application value is wide.