The effect of polyclonal antibody on intracellular calcium increase induced by Mycoplasma hyopneumoniae in porcine tracheal cells |
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Authors: | Hwang Mi-Hyun Lim Jong-Hwan Yun Hyo-In Kim Jong-Choon Jung Byeong-Yeal Hsu Walter H Park Seung-Chun |
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Institution: | Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea. |
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Abstract: | An investigation was undertaken to assess whether polyclonal convalescent and hyperimmune sera obtained from pigs inhibit Mycoplasma hyopneumoniae induced increases in intracellular calcium Ca2+](i) in ciliated porcine tracheal cells. Basal Ca2+](i) in the tracheal cells was 97+/-13 nM (n=22 cells in four experiments) and after exposure to M. hyopneumoniae (300 micro g/mL or 10(11) CCU/mL), Ca2+](i) increased by 246+/-56 nM within 100 s. After pre-treatment with hyperimmune or convalescent serum, M. hyopneumoniae increased Ca2+](i) by 196+/-43 and 223+/-65 nM, respectively. It was found that neither hyperimmune nor convalescent serum significantly prevented the increase in Ca2+](i) compared with M. hyopneumoniae alone. It was concluded that polyclonal antibodies produced by mycoplasma vaccination or exposure to the pathogen do not prevent M. hyopneumoniae-induced increase in Ca2+](i). |
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Keywords: | Calcium Mycoplasma hyopneumoniae Polyclonal antibody Porcine Trachea cell |
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