| Inactivation of Antinutritional Factors in Paseolus Vulgris by Proteases and Their Combinations with α-Deglycosidase |
| |
| 引用本文： | HuoGuicheng V.R.Fowler 等.Inactivation of Antinutritional Factors in Paseolus Vulgris by Proteases and Their Combinations with α-Deglycosidase[J].东北农业大学学报(英文版),1999,6(1):41-46. |
| |
| 作者姓名： | HuoGuicheng V.R.Fowler |
| |
| 作者单位： | [1]AnimalNutritionInstitute,NortheastAgriculturalUniversity,Harbin,PRC [2]ScottishAgriculturalCollege,581KingStreet,Aberdeen,AB9IUD |
| |
| 摘 要： | Aseries of in vitro trials was carried out to examine the capacity of eight proteolytic enxymes,which are either of bacterial or of fungal origins,and their combinations with a-deglycosidase in denaturing the activity of lectin and trypsin inhibitors in Paseolus vulgris,seed type “processor”,under the similar conditions to those of the gut.Znzymes,P3 and P4,were the most effective of hydrolyisis of trypsin inhibitory activity.After two hours incubation,trypsin inhibitory activity was reduced to 50 and 40% of the original value by P3 and P4 respectively.The rests of enzymes had little effects.Ompared with trypsin inhibitors,lectin was more resistant to hydrolysis.Only P4 reduced it by 26% of the original value.Pre-incubation with α-deglycosidase at its optimum conditions for one hour had no significant benefits for the tested proteases.
|
| 关 键 词： | 菜豆 抗营养因子 灭活 蛋白酶 α脱糖苷酸 |
Inactivation of Antinutritional Factors in Paseolus Vulgris by Proteases and Their Combinations with α-Deglycosidase |
| |
| Huo Guicheng,Yang Lijie,V.R.Fowler,M.Bedford,A.Morgan.Inactivation of Antinutritional Factors in Paseolus Vulgris by Proteases and Their Combinations with α-Deglycosidase[J].Journal of Northeast Agricultural University,1999,6(1):41-46. |
| |
| Authors: | Huo Guicheng Yang Lijie VRFowler MBedford AMorgan |
| |
| Abstract: | A series of in vitro trials was carried out to examine the capacity of eight proteolytic enxymes, which are either of bacterial or of fungal origins, and their combinations with adeglycosidase in denaturing the activity of lectin and trypsin inhibitors in Paseolus vulgris, seed type Processor,under the similar conditions to those of the gut. Enzymes, P3 and P4, were the most effective on hydrolyisis of trypsin inhibitory activity. After two hours incubation, trypsin inhibitory activity was reduced to 50 and 40% of the original value by P3 and P4 respectively. The rests of enzymes had little effects. Compared with trypsin inhibitors, lectin was more resistant to hydrolysis. Only P4 reduced it by 26% of the original value. Preincubation with deglycosidase at its optimum conditions for one hour had no significant benefits for the tested proteases. |
| |
| Keywords: | Phaseolus vulgris beans enzymes deglycosidase proteolysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
