(1) Instituto de FitopatologÍa y FisiologÍa Vegetal-INTA, Camino 60 cuadras km. 5 1/2 5119, CÓrdoba, Argentina;(2) Present address: Institute of Japan Plant Protection Associacion, Ibaraki, Japan
Abstract:
The rapid immunofilter paper assay (RIPA) was developed to detect Tomato spotted wilt virus (TSWV), Groundnut ringspot virus (GRSV), and Tomato chlorotic spot virus (TCSV) using antisera against recombinant nucleocapsid (N) proteins of each tospovirus. The two-step RIPA was sensitive enough to detect each pecies specifically in only 30min. This technique is proposed as an excellent tool for routine Tospovirus diagnosis and field epidemiological studies.