Diagnosis of three <Emphasis Type="Italic">Tospovirus</Emphasis> species by rapid immunofilter paper assay期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Diagnosis of three <Emphasis Type="Italic">Tospovirus</Emphasis> species by rapid immunofilter paper assay

Authors:	Paola López?Lambertini Laura?Williams Kenichiro?Shohara Email author" target="_blank">Daniel?Ducasse Email author

Institution:	(1) Instituto de FitopatologÍa y FisiologÍa Vegetal-INTA, Camino 60 cuadras km. 5 1/2 5119, CÓrdoba, Argentina;(2) Present address: Institute of Japan Plant Protection Associacion, Ibaraki, Japan

Abstract:	The rapid immunofilter paper assay (RIPA) was developed to detect Tomato spotted wilt virus (TSWV), Groundnut ringspot virus (GRSV), and Tomato chlorotic spot virus (TCSV) using antisera against recombinant nucleocapsid (N) proteins of each tospovirus. The two-step RIPA was sensitive enough to detect each pecies specifically in only 30min. This technique is proposed as an excellent tool for routine Tospovirus diagnosis and field epidemiological studies.

Keywords:	Rapid immunofilter paper assay (RIPA) Tospovirus diagnosis TSWV GRSV TCSV
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