Multiplex PCR for the identification of Agrobacterium biovar 3 strains |
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| Authors: | Akira Kawaguchi Hiroyuki Sawada Kouji Inoue Hideo Nasu |
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| Institution: | (1) Laboratory of Plant Pathology and Entomology, Agricultural Experiment Station, Okayama Prefectural General Agriculture Center, Sanyo-cho, Okayama 709-0801, Japan;(2) National Institute for Agro-Environmental Sciences, Tsukuba, Japan |
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| Abstract: | A biovar 3-specific primer set Ab3-F3/Ab3-R4 was designed based on the comparison of sequences of the 16S rDNA region of agrobacteria and related rhizobia for rapid identification of Agrobacterium biovar 3 strains. A 570-bp 16S rDNA fragment was amplified from cell lysates of Agrobacterium biovar 3 strains by polymerase chain reaction (PCR) using Ab3-F3/Ab3-R4 primers. Discrimination of Agrobacterium tumefaciens biovar 3 from Agrobacterium radiobacter biovar 3 and of Agrobacterium biovar 3 strains from other Agrobacterium strains was done simultaneously using multiplex PCR with a mixture of two primer sets (Ab3-F3/Ab3-R4 and VCF3/VCR3) previously designed for the virC region of Ti-plasmid and Ri-plasmid. |
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| Keywords: | Identification of Agrobacterium tumefaciens biovar 3 Multiplex PCR Crown gall of grapevine |
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