| 新型选择性培养基检测土壤棉花黄萎病菌效果初报 |
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| 引用本文： | 贾 涛,杨家荣. 新型选择性培养基检测土壤棉花黄萎病菌效果初报[J]. 勤云标准版测试, 2005, 0(5) |
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| 作者姓名： | 贾 涛 杨家荣 |
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| 作者单位： | 西北农林科技大学植物保护学院 陕西杨凌712100(贾涛)，西北农林科技大学植物保护学院 陕西杨凌712100(杨家荣) |
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| 基金项目： | 欧盟科技合作项目资助(ICA4-CT-20041-10001)。 |
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| 摘 要： | 介绍了一种用于检测土壤棉花黄萎病菌的选择性培养基。该培养基成份为:鲜棉叶100g,蔗糖5g,琼脂17~20g,40%PCNB0.3g,氯霉素、链霉素和青霉素各0.05g,水1000ml,pH6.4~7.0。该培养基与其它常见分离培养基相比,成份简单、操作简便,检测率显著,抑菌效果好,病菌在其上生长快、菌落典型,是一种较为理想的选择性分离培养基。
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| 关 键 词： | 土壤棉花黄萎病菌 选择性培养基 检测 |
A New Selective Medium to Be Used to Simply and Quick IsolateVerticillium dahliae in Cotton from Naturally Infested Soil |
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| Jia Tao,Yang Jiarong. A New Selective Medium to Be Used to Simply and Quick IsolateVerticillium dahliae in Cotton from Naturally Infested Soil[J]. , 2005, 0(5) |
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| Authors: | Jia Tao Yang Jiarong |
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| Affiliation: | (College of plant protection, North-west A and F University, Yangling 712100) |
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| Abstract: | A new selective medium to be used to simply and quick isolate Verticillium dahliae in cotton from naturally infested soil was introduced. The medium consisted of following ingredients per liter distilled water: leaves cotton 100g, sugar 5g, agar 11~20g, 40% PCNB 0.3g. chloramphenicol 0.05g, streptomycin 0.05g and penicillin 0.05g, pH adjusted to 6.4~7.0. The medium is simple and quick than old that. The microscclerotia V. dahliae from naturally infested soil could be effectively isolated while it was rarely infected by other pathogens. The colonies of V. dahliae on the medium were large black-colored. |
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| Keywords: | Verticillium dahliae in cotton Selective medium Isolating |
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