| 甘蓝型油菜花茎高效再生体系研究 |
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| 引用本文： | 韩德俊,曹 莉,袁本威,陈耀锋,李振岐. 甘蓝型油菜花茎高效再生体系研究[J]. 勤云标准版测试, 2005, 0(6) |
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| 作者姓名： | 韩德俊 曹 莉 袁本威 陈耀锋 李振岐 |
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| 作者单位： | 西北农林科技大学农学院 陕西杨凌712100(韩德俊，曹莉，袁本威，陈耀锋)，西北农林科技大学植物保护学院 陕西杨凌712100(李振岐) |
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| 基金项目： | 陕西省农业分子生物学实验室资助项目，杨凌基金资助项目 |
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| 摘 要： | 研究了甘蓝型油菜花茎外植体高效诱导不定芽的再生体系,结果表明:以含有2,4-D(0.5￣1.0mg/L)的培养基对外植体进行预培养,以及在分化培养基中加入AgNO3(2￣6mg/L),可显著降低外植体褐化坏死的频率,提高了不定芽的发生频率及其再生能力;6BA(2.5mg/L)与NAA(0.1mg/L)配合使用有利于提高不定芽发生频率;再生的不定芽90%可长根。
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| 关 键 词： | 甘蓝型油菜 花茎 不定芽再生 2 4-D AgNO3 |
Efficient Regeneration from Peduncle Segments of Brassica napus L. |
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| Han Dejun,Cao Li,Yuan Benwei,Chen Yaofeng,Li Zhenqi. Efficient Regeneration from Peduncle Segments of Brassica napus L.[J]. , 2005, 0(6) |
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| Authors: | Han Dejun Cao Li Yuan Benwei Chen Yaofeng Li Zhenqi |
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| Affiliation: | (1College of Agronomy, Northwest A&F University, YangLing Shaanxi 712100; 2College of Plant Protect, Northwest A&F University, YangLing Shaanxi 712100) |
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| Abstract: | Excised peduncle segments of three Brassica napus L. cultivars were used to optimize conditions for high-frequency shoot regeneration. The presence of 2,4-D(0.5~1.0 mg/L) in pre-cultured medium markedly enhanced the subsequent frequency of responding explants and the number of shoots/responding explant. The use of AgNO3 was a prerequisite for efficient shoot regeneration due to it strongly increased morphogenesis and decrease the frequency of explants death. BA (2.5mg/L) in combination with a low concentration of NAA(0.1mg/L) gave optimum shoot bud differentiation. The regenerated shoots could be rooted at a frequency of 95%. |
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| Keywords: | Brassica napus Peduncle segments Shoot regeneration 2 4-D AgNO3 |
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