野生‘沉香虎头兰’种子无菌萌发及快速繁殖技术研究 |
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作者姓名: | 李枝林 余朝秀 王玉英 王卜琼 |
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作者单位: | 云南农业大学花卉研究所 昆明650201
(李枝林,余朝秀,王玉英),云南农业大学花卉研究所 昆明650201(王卜琼) |
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基金项目: | 国家自然科学基金资助项目(30160074);云南省科学基金重点项目(2002C003P). |
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摘 要: | 对沉香虎头兰种子进行无菌萌发和快繁研究,结果表明:1/2Ms 6-BA1.0-2.0mg/L NAA1.0-2.0mg/L可使种子萌发率达95%以上,效果最好;原球茎培育以1/2MS KT2.0mg/L NAA0.5mg/L为好,增殖培养以1/2MS 6-BA3.0mg/L NAA0.2mg/L最好;而生根培养以10-6Y2注]为宜。
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关 键 词: | 沉香虎头兰 种子 无菌培养 快繁 |
esearch on Aseptic Budding and Fast Propagation
of the Cymbidium tracyanum Seed |
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Authors: | Li Zhilin Yu Chaoxiu Wang Yuying Wang Boqiong |
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Institution: | (Floral Research Institute of Yunnan Agricultural University, Kunming 650201) |
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Abstract: | Research for the aseptic budding and the fast propagation of wild Cymbidium tracyanum L castle seed indicates that: the seed budding rate reaches above 95% and has the best result if culture and hormone of 1/2 Ms 6-BA 1.0~2.0mg/L NAA 1.0~2.0mg/L are taken; and for original corm breeding, 1/2 MS KT2.0mg/L NAA0.5mg/L is better; for seed reproduction culture, 1/2 MS 6-BA 3.0 mg/L NAA mg/L is suitable; while for rhizogenic culture, better use 10-6Y2. |
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Keywords: | Cymbidium tracyanum Seed Aseptic culture Fast propagation |
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