| 绵羊卵母细胞OPS玻璃化冷冻效果初报 |
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| 作者姓名： | 张晓建 陈静波 张涌等 |
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| 作者单位： | 西北农林科技大学生物工程研究所，新疆天康畜牧技术股份有限公司胚胎生物工程部，西北农林科技大学生物工程研究所 陕西杨凌712100，乌鲁木齐830000，新疆畜牧科学院，乌鲁木齐830000，陕西杨凌712100 |
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| 基金项目： | 新疆科技厅“胚胎体外高效培养系统及胚胎性别鉴定研究”重点项目资助。 |
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| 摘 要： | 比较了绵羊卵母细胞不同发育阶段对OPS玻璃化冷冻效果的影响,同时比较了程序化冷冻与OPS玻璃化冷冻的效果。结果显示:成熟培养0h(GV期)、16h、24h、26h的卵母细胞玻璃化冷冻、解冻后形态正常数差异不显著()P<0.05),培养24h卵母细胞冷冻保存后,分裂卵数、囊胚发育数与培养0h、16h、26h试验组差异显著(38.3%VS13.3%、26.7%、28.3%;11.7%VS0%、5.0%、8.3%,P<0.05),而培养16h、26h两个试验组之间差异不显著(P<0.05);程序化冷冻方法(参考胚胎程序化冷冻)、OPS玻璃化冷冻与不冷冻三组试验在形态正常数、分裂卵数和囊胚发育数上差异都极显著(20.0%VS54.1%、100%;6.7%VS37.5%、78.3%;0%VS11.4%、23.3%,P<0.01)。
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| 关 键 词： | 绵羊 卵母细胞 OPS玻璃化冷冻 |
Vitrification of Sheep Oocyte s by Open Pulled Straw |
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| Authors: | Zhang Xiaojian Chen Jinbo Zhang Yong |
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| Abstract: | The objective of this study was to compare the effects of different development stage of oocyte and different fundamental medium on the development capacity of OPS vitrifying sheep oocytes, and compared the effects of conventional preservation and OPS vitrification. The results show: all groups of different development stage of oocyte have no difference on the rate of morphologically normal,24h group had a higher rate of cleavage and blastocyst than other groups(38.3%VS 13.3%?26.7%?28.3%;11.7%VS 0%?5.0%?8.3%, P < 0. 05),but there had no difference between 18h group and 26h group(P<0. 05); The conventional preservation has a lower rate than OPS vitrification and control(20.0% VS 54.1%, 100%; 6.7% VS 37.5%, 78.3%;0% VS 11.4%, 23.3%, P <0.01). |
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| Keywords: | Sheep Oocyte OPS Vtrification |
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