| 抗雌二醇单克隆抗体的制备与鉴定 |
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| 引用本文： | 戴小华,吴国娟,沈 红,赵小萌,陆 彦. 抗雌二醇单克隆抗体的制备与鉴定[J]. 勤云标准版测试, 2005, 0(6) |
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| 作者姓名： | 戴小华 吴国娟 沈 红 赵小萌 陆 彦 |
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| 作者单位： | 北京农学院,北京农学院,北京农学院,北京农学院,北京农学院 北京102206新疆农业大学,乌鲁木齐830052,北京102206,北京102206,北京102206,北京102206新疆农业大学,乌鲁木齐830052 |
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| 基金项目： | 北京市农委科技攻关资助项目"畜产品中药物残留快速检测技术及产品研究与开发"(20020103). |
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| 摘 要： | 活化雌二醇(E2)C3处的酚-OH,与牛血清白蛋白(BSA)偶联,制备得E2的完全抗原,利用完全抗原免疫动物,采用单克隆抗体技术,经过细胞融合、克隆筛选、扩大培养、动物体内诱生腹水等过程,最后获得了E2的单克隆抗体,对纯化的单克隆抗体的性质鉴定,结果显示:其抗体效价为1:106,抗体属于IgG2a型;分子量为179000Da;E2单克隆抗体的亲和常数K2.9108L/mol。
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| 关 键 词： | 雌二醇 细胞融合 单克隆抗体 |
Production and Characterization of Anti-estradiol Monoclonal Antibody |
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| Dai Xiaohu,Wu Guojuan,Shen Hong,Zhao Xiaomeng,Lu Yan. Production and Characterization of Anti-estradiol Monoclonal Antibody[J]. , 2005, 0(6) |
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| Authors: | Dai Xiaohu Wu Guojuan Shen Hong Zhao Xiaomeng Lu Yan |
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| Affiliation: | (1Beijing Agriculture College, Beijing 102206; 2Xinjiang Agricultrue University, Urumqi Xinjiang 830052) |
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| Abstract: | Estradiol (E2) was bound to bovine serum albumin (BSA) to form a complete antigen (E2-BSA), after the hydroxy (-OH) at the position of C3 was activated. A BALB/c mouse was immunized with the mixture of E2-BSA and Freund adjuvant. Monoclonal antibody-producing hybridoma anti-E2 was prepared by fusion of SP2/0 murine myeloma cells with spleen cell isolated from the immunized mouse. A monoclonal antibody (McAb) specific for E2 was produced in vivo by the hybridoma anti-E2. Analysis revealed that anti-E2 McAb (E2-McAb) was of the IgG2a type, with the molecular weight179000 daltons. The titer of E2-McAb was 1:106, and the affinity constant of constant of E2-McAb was 2.9×108L/mol. |
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| Keywords: | Estradiol Cell fusion Monoclonal antibody |
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