| 小麦蓝矮病植原体的分子生物学检测研究 |
| |
| 引用本文： | 张 荣,孙广宇,张雅梅,张秦风,赵二龙. 小麦蓝矮病植原体的分子生物学检测研究[J]. 勤云标准版测试, 2005, 0(8) |
| |
| 作者姓名： | 张 荣 孙广宇 张雅梅 张秦风 赵二龙 |
| |
| 作者单位： | 西北农林科技大学植保学院 陕西杨凌712100(张荣，孙广宇，张雅梅，张秦风)，西北农林科技大学资环学院 陕西杨凌712100(赵二龙) |
| |
| 基金项目： | 陕西省自然科学基金项目(99SM03) |
| |
| 摘 要： | 小麦蓝矮病是中国北方小麦的重要病害。利用植原体特异引物对小麦蓝矮病带病植株总DNA进行巢式-PCR(Nested-PCR)扩增,获得1.2kb的特异性片段,从分子水平上证明小麦蓝矮病是一种植原体病害。对具有红矮和蓝矮症状的病株扩增均可扩增出目标片段,说明红矮和蓝矮症状都是由植原体引起。试验为在小麦蓝矮病分子诊断、田间介体带毒检测、寄主范围研究等提供一种快速、灵敏可靠的方法。
|
| 关 键 词： | 小麦 小麦蓝矮病 植原体 巢式PCR |
Molecular Detection of Phytoplasma associated with Wheat Blue Dwarf |
| |
| Zhang Rong,Sun Guangyu,Zhang Yamei,Zhang Qinfeng,Zhao Erlong. Molecular Detection of Phytoplasma associated with Wheat Blue Dwarf[J]. , 2005, 0(8) |
| |
| Authors: | Zhang Rong Sun Guangyu Zhang Yamei Zhang Qinfeng Zhao Erlong |
| |
| Affiliation: | (1College of Plant Protection, Northwest A & F University, Yangling Shaanxi 712100;2College of Resources and Environments, Northwest A & F University, Yangling Shaanxi 712100) |
| |
| Abstract: | Wheat Blue Dwarf is one of the main disease on winter wheat in Northwestern China. A 1.2kb fragment was amplified by phytoplasmas universal primers in total DNA sample extracted from diseased wheat with dark green leaves and red dwarf symptom by Nested-PCR. The result indicated molecularly that the pathogen of Wheat Blue Dwarf disease was phytoplasma. The different symptoms (dark green dwarf and red dwarf) were all caused by phytoplasmas. Nested-PCR could be as a rapid, exact technique for detecting the pathogens from wheat leaves, insect vectors and weed host. |
| |
| Keywords: | Triticum aestivum L Dwarf Phytoplasmas Nested-PCR |
|
| 点击此处可从《勤云标准版测试》浏览原始摘要信息 |
|
点击此处可从《勤云标准版测试》下载全文 |
|
