大鼠慢性硒中毒的病理形态学研究

PATHOMORPHOLOGICAL STUDIES ON CHRONIC SELENIUM POISONING IN WESTAR RATS

Changes of pathomorphology in experimental chronic selenosis of Wister rats hadbeen studied. Forty six male wister rats, weighing 105-135 grams were divided intotwo groups. Twenty eight rats in the experimental group were fed basal diet supplem-ented with 10 ppm selenium (se) of sodium selenite, while the eighteenrats of controlgroup were only fed with basal diet. Growth performances of rats, the ratio of liver,kidney, spleen weight per 100 gm of body were determined. On necropsy, both grossand histopathological lesions in liver, kidney, spleen and lymph nodes were studied Growth rate in the experimental group was significantly depressed (P<0. 001).Liver and bodyweight ratio was increased (P<0. 01), while kidney and spleen withthe ratio of body weight were increased only at the advanced stage. Gross and histopathological examination revealed that there were marked damagesin liver, kidney, spleen and lymph nodes. Characteristic lesions in the liver wereintralalobular venules and capillary congestion, hepatocellular degeneration and necrosisin early stage. At the advanced stage, epithelium of intrahepatic bile duct proliferationand heptocyte regeneration were the prominent changes. In kidney, there were tubularcell degeneration and necrosis. The major histopathological findings in the spleen.Lymphocytes of white pulp were sparsely distributed, but in the advaned stage thedegree of severity became to be reduced. Hemorrhages and degeneration were commonlesions in lymph nodes. Also, lymph node lesions were gradually replaced by chronicproliferation lymphadenitis.