| 小麦“5389”悬浮细胞系建立的条件摸索 |
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| 引用本文： | 乔妹,陈琰,张洁,王冬梅. 小麦“5389”悬浮细胞系建立的条件摸索[J]. 河北农业大学学报, 2010, 33(6) |
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| 作者姓名： | 乔妹 陈琰 张洁 王冬梅 |
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| 作者单位： | 河北农业大学,生命科学学院,河北,保定,071001 |
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| 基金项目： | 国家自然科学基金(30671244); 河北省应用基础研究计划重点基础研究项目(08965505D); 河北省自然科学基金(303180,C2005000220,C2007000515,C2010000787)资助 |
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| 摘 要： | 以小麦"5389"的幼胚为外植体诱导愈伤组织,研究了培养基中2,4-D、KT、ABA及其配比对愈伤组织诱导和愈伤组织继代培养的影响。结果表明:以MS+2,4-D 3.0 mg/L培养基的诱导效果最好,出愈率最高达93.42%;在MS+2,4-D 1.0 mg/L+KT 0.1 mg/L培养基上进行继代培养获得松脆型愈伤组织,以MS+2,4-D1.0 mg/L+KT 0.1 mg/L培养基进行液体培养建立了状态良好的悬浮培养细胞。
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| 关 键 词： | 小麦 幼胚 愈伤组织 悬浮培养 |
Conditions for establishment of cell suspension culture of wheat 5389 |
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| QIAO Mei,CHEN Yan,ZHANG Jie,WANG Dong-mei. Conditions for establishment of cell suspension culture of wheat 5389[J]. Journal of Agricultural University of Hebei, 2010, 33(6) |
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| Authors: | QIAO Mei CHEN Yan ZHANG Jie WANG Dong-mei |
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| Affiliation: | QIAO Mei,CHEN Yan,ZHANG Jie,WANG Dong-mei(College of Life Science,Agricultural University of Hebei,Baoding 071001,China) |
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| Abstract: | Rataria of wheat 5389 were cultured to study the effect of different mediums on callus induction and the estate of the callus.The results showed that in MS+2,4-D 3.0 mg/L culture medium the highest ratio of callus induced was 93.42%,and in MS+2,4-D 1.0 mg/L+KT 0.1 mg/L maintenance medium the embryonal callus were obtained and cell suspension culture of wheat 5389 was established.These results provided a good system for further study on how the elicitor can induce the defense reaction of host. |
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| Keywords: | wheat rataria callus suspension culture |
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