| 大叶黄杨带腋芽茎段组织培养研究 |
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| 引用本文： | 吴广宇,杨玲玲.大叶黄杨带腋芽茎段组织培养研究[J].安徽农业科学,2005,33(5):815-815,817. |
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| 作者姓名： | 吴广宇 杨玲玲 |
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| 作者单位： | 长江大学园艺园林学院,湖北,荆州,434025;长江大学园艺园林学院,湖北,荆州,434025 |
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| 摘 要： | 以大叶黄杨带腋芽茎段为材料,以MS和1/2MS为基本培养基,进行组织培养研究。结果表明:以pH值6.2的MS+6BA2.0mg/L为培养基,用浓度0.1%升汞溶液消毒大叶黄杨带腋芽茎段5min,其试管苗生长良好,成活率可达93%;生根培养基采用1/2MS+NAA1mg/L+0.5%活性炭,生根率可达95%。
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| 关 键 词： | 大叶黄杨 茎段 组织培养 |
| 文章编号： | 0517-6611(2005)05-0815-01 |
Study on Stem Segment with Axillary's Bud Culture of Euonymus japonica us |
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| WU Guang-yu et al.Study on Stem Segment with Axillary's Bud Culture of Euonymus japonica us[J].Journal of Anhui Agricultural Sciences,2005,33(5):815-815,817. |
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| Authors: | WU Guang-yu |
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| Abstract: | Euonymus japonica us stem segment with axillary's bud was cultured with MS and 1/2MS medium in this experiment. The results showed that MS+6-BA 2.0?mg/L culture medium and taking antisepsis for stem segment with axillary's bud with 0.1%HgCl_2 five minutes were the best. And in the tube culture the plantlets grew rapidly. The survival rate of transplanting reached to 93% and the plantlets in tube was cultured in 1/2MS+NAA?1? mg/L +0.5% active carbon and the young shoot will reach to 95%. |
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| Keywords: | Euonymus japonica us Stem segment with axillary's bud culture |
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