基于高通量测序白及根部内生真菌的物种组成分析

从云南省南华县采集不同地理位置的白及植株样品共3个（HCN 1、HCN 2、HCN 3），并记录和采集样品区域的生境和根部土壤。通过高通量测序技术，分析白及根部内生真菌物种组成差异，进而对白及根部内生真菌中有益菌株进行筛选。研究表明：Phyllosticta sp.，Erysiphe sp.，Podosphaera sp.，Glomerella cingulata，Aspergillus sp.，Rhodosporidium diobovatum，Sterigmatomyces halophilus在各采样点的物种组成差异明显。白及根部内生真菌中，Phyllosticta sp.，Erysiphe sp.，Podosphaera sp.，Glomerella cingulata是病原菌，Aspergillus sp.，Rhodosporidium diobovatum，Sterigmatomyces halophilus是已明确的生防菌，白及植株根部并未表现出明显的患病特征，与同时存在具有生防功能的菌属丰度之间有着密切联系。采样距离、地理位置差异、土壤理化性质均是引起白及根部内生真菌组成差异的影响因子，其中土壤理化性质是白及根部内生真菌物种组成差异的主要影响因素。

Analysis of Species Composition of Endophytic Fungi in Roots of Bletilla striata Based on High Throughput Sequencing

In this paper, 3 plant samples of Bletilla striata from diferent sites were collected in Nanhua County, Yunnan Province (HCN 1, HCN 2, HCN 3), and respective habitat recorded and soil sampled. The different species composition of endophytic fungi in the roots of B. striata was analyzed by high throughput sequencing technique. The results showed that Phyllosticta sp., Erysiphe sp., Podosphaera sp., Glomerella cingulata, Aspergillus sp., Rhodosporidium diobovatum and Sterigmatomyces halophilus were significantly different in species abundance and composition among sampling sites. It is speculated that Phyllosticta sp., Erysiphe sp., Podosphaera sp. and Glomerella cingulate were pathogenic fungi, whilst Aspergillus sp., Rhodosporidium diobovatum and Sterigmatomyces halophilus well-defined biocontrol fungi. The root of B. striata did not show visible disease symptoms possibly due to the existence of biocontrol fungi. In this study, sample distance, geographical location, and soil physical and chemical properties are the factors responsible for the difference in endophytic fungi composition in roots of B. striata. Among which, soil physical and chemical properties are the key factors.