| 贺兰山野生岩羊布鲁氏菌病分子生物学检测与分析 |
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| 引用本文： | 孙静,马泽林,刘建丽,金红梅,张小林,郑伟杰,柴洪亮,华育平. 贺兰山野生岩羊布鲁氏菌病分子生物学检测与分析[J]. 安徽农业科学, 2014, 42(36): 12935-12936,12946 |
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| 作者姓名： | 孙静 马泽林 刘建丽 金红梅 张小林 郑伟杰 柴洪亮 华育平 |
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| 作者单位： | 东北林业大学野生动物资源学院,黑龙江哈尔滨,150040;东莞出入境检验检疫局,广东东莞,523072 |
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| 基金项目： | 中央高校基本科研业务费资助项目，野生动物国家级实验教学示范中心建设项目 |
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| 摘 要： | [目的]了解宁夏银川贺兰山部分区域岩羊布鲁氏菌病的分布情况.[方法]以提取53份岩羊样品DNA为模板,使用P1和P2引物进行PCR扩增,对贺兰山野生岩羊布鲁氏菌病的分布状况进行检测.[结果]53份野生岩羊样品中布鲁氏菌病的阳性检出率为0.[结论]该研究表明宁夏贺兰山野生岩羊中不存在布鲁氏菌病,但还需要进一步研究.
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| 关 键 词： | 岩羊 布鲁氏菌病 分子生物学 检测 |
Molecular Biology Detection and Analysis of Brucellosis in Wild Blue Sheep in Helan Mountains |
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| SUN Jing;MA Ze-lin;LIU Jian-li;CHAI Hong-liang;HUA Yu-ping. Molecular Biology Detection and Analysis of Brucellosis in Wild Blue Sheep in Helan Mountains[J]. Journal of Anhui Agricultural Sciences, 2014, 42(36): 12935-12936,12946 |
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| Authors: | SUN Jing MA Ze-lin LIU Jian-li CHAI Hong-liang HUA Yu-ping |
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| Affiliation: | SUN Jing;MA Ze-lin;LIU Jian-li;CHAI Hong-liang;HUA Yu-ping;College of Wildlife Resources,Northeast Forestry University;Dongguan Entry-exit Inspection and Quarantine Bureau; |
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| Abstract: | [Objective] The research aimed to understand the distribution situations of brucellosis in blue sheep in some regions of HelanMountains in Yinchuan of Ningxia. [ Method ] 53 DNA samples extracted from blue sheep were taken as template to make PCR amplificationwith P1 and P2 primers. The distribution situations of brucellosis in blue sheep in Helan Mountains were detected. [ Result] The positive rateof brucellosis in 53 samples of blue sheep was zero. [ Conclusion] The research results showed that no brucellosis was detected in these bluesheep, but it needed to be further studied. |
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| Keywords: | Blue sheep Brucellosis Molecular biology Detection and analysis |
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