天蓝苜蓿组织培养及再生体系的研究

以野生天蓝苜蓿种子发育5～7d无菌苗的子叶、下胚轴和种子苗叶片为外植体,对其愈伤组织诱导及其分化的基本培养与外源激素组合和浓度配比进行试验。结果表明:对子叶、下胚轴和叶片愈伤组织诱导最适宜的基础培养基分别是MS、B5和SH;激素的种类及其浓度配比对于愈伤组织的诱导因不同的外植体有很大的差异。其中,子叶、下胚轴以MS+NAA(0.5～1.0 mg/L)+6-BA(0.7mg/L)效果较好,叶片以SH+NAA(0.5～1.0mg/L)+2,4-D(1.5～5 mg/L)+6-BA(0.7mg/L)较好,少量下胚轴在MS+NAA(0.5mg/L)+6-BA(0.7mg/L)和MS+NAA(0.5mg/L)+KT(0.5 mg/L)的培养基中直接分化出胚状体,并成功发育成植株。在培养基Whb5上,子叶、下胚轴和叶片的分化都得到了很好的效果,分化率分别为43%、57%、40%,15～30d后都能再生植株。

Study on the Tissue Culture and Regeneration System of Medicago lupulina L.

Cotyledon,hypocotyls and leaf of 5～7d's seedling after germination were used as explants to study the effect of different basic media on callus induction and the combinative concentration of hormones on their differentiation.The results showed that MS?B5 and SH were the optimum basic media for the callus induction of cotyledon,hypocotyls and leaf,respectively.The combinative concentrations of hormones showed very different among different explants.The combinative concentration of hormone MS+NAA(0.5～1.0mg/L)+6-BA(0.7mg/L) was suitable for cotyledon and hypocotyls,and SH+ NAA(0.5～1.0mg/L)+2,4-D(1.5～5 mg/L)+ 6-BA(0.7mg/L) for leaf.A few of hypocotyls were differentiated embryoid directly,moreover developed plantlet in the media of MS+NAA(0.5mg/L)+6-BA(0.7mg/L) and MS+NAA(0.5mg/L)+KT(0.5mg/L).In the progress of differentiation,cotyledon,hypocotyls and leaf showed better effect in the medium whb5,differentiating rate of green callus up to 43%,57% and 40% respectively,and all could regenerate plantlet after 15～17days.