| 鸡睾丸生精上皮细胞冷冻保存研究 |
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| 引用本文： | 采克俊,张易祥,丁志丽,谢小丹,周源,刘莉. 鸡睾丸生精上皮细胞冷冻保存研究[J]. 黑龙江畜牧兽医, 2009, 0(3) |
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| 作者姓名： | 采克俊 张易祥 丁志丽 谢小丹 周源 刘莉 |
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| 作者单位： | 湖州师范学院生命科学学院,浙江湖州313000 |
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| 基金项目： | 国家自然科学基金项目(30500270);;浙江省科技攻关重点计划项目(2005C22052);;浙江省教育厅科研项目(20070479);;湖州市自然科学基金项目(2006YZ08) |
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| 摘 要： | 以广西土鸡为试验材料，利用两步酶解法从出壳后20～30日龄公鸡睾丸中获取生精上皮细胞，以含20％胎牛血清的DMEM为基础培养液，比较了不同浓度DMSO和蔗糖对生精上皮细胞冷冻保存效果的影响。结果表明：DMSO的最佳浓度为10％，添加蔗糖对冻存有害。以10％DMSO为防冻剂，复苏后生精上皮细胞原代混合培养可形成典型的精原干细胞集落，集落呈碱性磷酸酶（AKP）阳性，说明以10％DMSO为防冻剂的冷冻体系适合鸡睾丸生精上皮细胞的冷冻保存。
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| 关 键 词： | 鸡 生精上皮细胞 精原干细胞 冷冻保存 体外培养 |
Study on cryopreservation of seminiferous epithelial cells in the testes of chicken |
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| CAI Ke-jun,ZHANG Yi-xiang,LIU Li,et al. Study on cryopreservation of seminiferous epithelial cells in the testes of chicken[J]. Heilongjiang Animal Science And veterinary Medicine, 2009, 0(3) |
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| Authors: | CAI Ke-jun ZHANG Yi-xiang LIU Li et al |
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| Affiliation: | School of Life Sciences;Huzhou Teachers college;Huzhou 313000;China |
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| Abstract: | Chickens(from 20-day-old to 30-day-old male)were used to isolate seminiferous epithelial cells by sequential two-step enzyme digestion.Cells were cultured in DMEM supplemented with 20% fetal bovine serum,and the effects of dimethyl sulfoxide(DMSO) and sucrose with different concentrations were tested.The results showed that the survival rate of cell was the highest in condition of 10% DMSO,whereas the addition of sucrose was harmful for the seminiferous epithelial cells.The seminiferous epithelial cells wer... |
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| Keywords: | Chicken seminiferous epithelial cells spermatogonial stem cells cryopreservation culture in vitro |
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