| 植物总DNA提取方法的改进 |
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| 引用本文： | 王文斗,段英俊,那冬晨.植物总DNA提取方法的改进[J].安徽农业科学,2013(24):9913-9913,9923. |
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| 作者姓名： | 王文斗 段英俊 那冬晨 |
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| 作者单位： | 山西师范大学生命科学学院,山西临汾,041004;山西师范大学生命科学学院,山西临汾,041004;山西师范大学生命科学学院,山西临汾,041004 |
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| 摘 要： | 目的]改进植物总DNA的提取方法.方法]采用2种研磨方式进行植物DNA提取效果的比较.结果]2种研磨方式所得DNA质量都较高,可用于后续分子生物学研究;采用研磨缓冲液提取的DNA得率高于液氮研磨.结论]该方法降低了DNA的提取成本,是一种经济实用的DNA提取方法.
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| 关 键 词： | DNA提取 研磨缓冲液 经济实用 |
Improvement of Extraction Method of Total DNA from Plant |
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| Institution: | WANG Wen-dou et al (College of Life Science, Shanxi Normal University, Linfen, Shanxi 041004) |
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| Abstract: | Objective ] The aim was to improve the extraction method of total DNA from plant. Method ] Two kinds of grinding method of DNA extraction were compared in extraction effects. Result ] The quality of DNA extracted by the two kinds of grinding method was good, and the DNA could be used in the follow - up molecular biology study ; the DNA extraction yield of grinding buffer grinding was higher than that of liquid nitrogen grinding. Conclusion ] The method could reduce the cost of DNA extraction, and was an economical and available method for DNA extraction. |
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| Keywords: | DNA extraction Grinding buffer Economical and available |
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