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| 晚熟甜橙芽变的ISSR鉴定 | |
| 引用本文： | 程昌风,何桥,洪林,郭启高,漆巨容,吴纯清,余德全,魏召新,梁国鲁. 晚熟甜橙芽变的ISSR鉴定[J]. 西南农业学报, 2008, 21(5) |
| 作者姓名： | 程昌风 何桥 洪林 郭启高 漆巨容 吴纯清 余德全 魏召新 梁国鲁 |
| 作者单位： | 1. 重庆市农科院果树研究所,重庆江津,402260 2. 西南大学园艺园林学院,重庆,400716 3. 重庆长寿区农业局果经站,重庆长寿,401220 |
| 摘 要： | 应用ISSR技术对晚熟甜橙伏令夏橙的大果无核枝、"安顺一号"等5个材料进行了鉴定。从100个引物中筛选出18个引物,共扩增出113条带,其中多态性条带数为15条,多态位点百分率为13.3%。结果表明:812、834、841引物对参试材料有明显扩增带差异,伏令夏橙大果无核枝与对照枝及伏令夏橙、"安顺一号"与伏令夏橙及锦橙在DNA分子水平上存在明显的遗传差异,晚熟甜橙大果无核枝为伏令夏橙一稳定芽变。ISSR技术能有效地鉴定晚熟甜橙芽变。 |
| 关 键 词： | 晚熟甜橙 变异 ISSR鉴定 |
The identification of Citrus sinensis cv.bud mutation by ISSR markers |
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| CHENG Chang-feng,HE Qiao,HONG Lin,GUO Qi-gao,QI Ju-rong,WU Chun-qing,YU De-quan,WEI Zhao-xin,LIANG Guo-lu. The identification of Citrus sinensis cv.bud mutation by ISSR markers[J]. Southwest China Journal of Agricultural Sciences, 2008, 21(5) | |
| Authors: | CHENG Chang-feng HE Qiao HONG Lin GUO Qi-gao QI Ju-rong WU Chun-qing YU De-quan WEI Zhao-xin LIANG Guo-lu |
| Abstract: | DNA polymorphism of C.sinensis cv.were determined by inter simple sequence repeat(ISSR) molecular markers.113 bands were amplified with 18 of 100 primers,among which 15 were polymorphic between mutants and parental cultivars,and the polymorphism ratio was 13.3%.These results showed that there were genetic diversity with primer 812,834 and 841 between seedless big fruit branch and the contrast branch,among 'Anshun No.1' and Valencia and 'Jincheng' orange.Combining the results of morphological and molecular analysis,it was certified that the branch of C.sinensis cv.and 'Anshun No.1' occurred genetic variation. |
| Keywords: | late orange bud mutation ISSR identification |
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