高邮鸭肌肉生长抑制素基因(MSTN)5'调控区多态性与生长及腹脂率的关联分析

肌肉生长抑制素(Myostatin,MSTN)基因在发育和成熟的骨骼肌中特异表达,对肌肉具有负调控作用。采用PCR-SSCP技术检测了3个不同高邮鸭群体和1个绍鸭群体MSTN5'的单核苷酸多态性,并与0及3～10周龄体重和腹脂率进行相关性分析。结果:在MSTN基因5'调控区的1 046 bp处检测到C→G的突变。在4个鸭群中,均检测到3种基因型(CC、CG和GG基因型),且都以C等位基因为优势等位基因。最小二乘法分析结果表明,不同基因型对试验的4个群体鸭周龄生长无显著影响;高邮鸭3个群体间平均腹脂率无显著差异,但绍鸭腹脂率均极显著低于3个高邮鸭群体(P<0.01);不同基因型对高邮鸭3个不同群体的腹脂率均有显著影响,GG型高邮鸭腹脂率显著高于CG型和CC型(P<0.05)。就5'调控区而言,MSTN基因作为影响腹脂率的候选基因有待于进一步的研究。

Association of the MSTN-5'regulatory region polymorphisms with body weights and abdominal fat rates in the Gaoyou duck

Single nucleotide polymorphisms of MSTN 5′regulatory region of 3 different Gaoyou and 1 Shaoxing duck populations were detected using PCR-SSCP.Genetic effects of the MSTN 5′regulatory region on body weights and abdominal fat rates were analyzed.The results showed that C-G of mutations in 1046st bp of MSTN gene promoters was detected.In the test of 4 duck populations,three genotypes(CC,CG and GG gene type) were detected,and with C alleles for advantage alleles.The least square analysis results showed that different genotypes had no significant effects on week body weights.In addition,no significant differences were found among abdominal fat rates of 3 Gaoyou duck populations,while average abdominal fat rates of 3 Gaoyou duck populations were significantly greater than that of Shaoxing duck population.Different genotypes had a significant effect on abdominal fat rates of Gaoyou ducks and abdominal fat rate of the GG genotype was significantly greater than that of CG and CC type(P<0.05).In regard to MSTN 5′regulatory region,MSTN gene,as a candidate gene of affecting abdominal fat rates,needs to be further researched.