造血和神经相关标志物在人胎盘的表达

为探讨人胎盘组织在造血中的作用以及研究造血和神经标志物在胎盘中的表达和在胚胎发育中的相关性，用免疫组织化学法对人胎盘组织和体外培养的胎盘组织中的贴壁细胞进行染色，观察其造血因子和神经标志物的表达。结果发现人7月龄胎盘组织血管内血细胞表达多种造血细胞因子，如SCF、VEGF、BMP-4和FGF-1和KDR，血管内皮细胞上也有表达；胎盘组织同时表达造血干细胞标记CD34、CD133和神经细胞标记物Nestin和MAP2等。而足月龄胎盘CD34、CD133、KDR和造血相关因子SCF、VEGF、BMP-4、FGF-1等弱表达，不表达Nestin和MAP2。两组胎盘切片都不表达GFAP和MBP。胎盘贴壁细胞（hPDACs）碱性磷酸酶、波形蛋白、CD133、Nestin和MAP2染色呈阳性，CD34、GFAP和MBP阴性表达。人胎盘组织和胎盘贴壁细胞表达多种造血相关因子和神经细胞标志物，提示胎盘具有造血功能，造血形成和神经发生之间可能存在基因表达叠加现象。

Research on Expression of Hematopoietic and Neural Markers in Human Placenta

The expression of neural and hematopoietic markers in placenta tissue and cultured human placenta derived adherent cells(hPDACs) and their correlation in embryonic development were investigated in this paper.The function of human placenta in hematopoiesis was demonstrated by immunocytological study in 7 and full-term months'placental slices.Positive reactions were observed in 7 month placenta using antibody against SCF,VEGF,BMP-4,FGF-1,CD34,CD133 and KDR.However,full-term months'placenta just reacted slightly with these antibodies.The staining for neural markers such as Nestin and MAP_2 showed positive reactions in 7 month and negative in full-term placenta.GFAP was showed negative in all two groups(placenta's) dying.The isolated adherent cells of placenta were positive for AKP,vitamin,SCF,VEGF,BMP-4, FGF-1,CD133,Nestin and MAP_2 markers,while they were negative for CD34,GFAP and MBP.They showed neural differentiation potential under appropriate conditions.The data suggest that placenta can express hematopoietic related factors to support hematopoiesis,and placental-derived cells have neural differentiation potential.