| 食源性沙门氏菌PCR检测体系的建立及评估 |
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| 引用本文： | 郑鸣,郑宝亮.食源性沙门氏菌PCR检测体系的建立及评估[J].郑州牧业工程高等专科学校学报,2010,30(1). |
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| 作者姓名： | 郑鸣 郑宝亮 |
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| 作者单位： | 郑州牧业工程高等专科学校,生物工程系,河南,郑州,450011 |
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| 摘 要： | 为建立食源性沙门氏菌实时定量PCR检测体系,实现快速检测。根据GenBank数据库鼠伤寒沙门氏菌的invA基因序列(AE008832)设计引物,建立沙门氏菌实时定量PCR检测方法,通过标准曲线的建立和对40份牛奶和40份生肉进行检测评估检测体系的灵敏度和特异性。结果显示标准曲线相关系数为0.999,检出底限约8个细菌/反应。40份牛奶和40份生肉检测阳性率分别为2.5%和5%,与传统细菌分离检测结果完全相符。实时定量PCR检测沙门氏菌具快速、灵敏的优点,适宜于食品中沙门氏菌污染的调查及监测。
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| 关 键 词： | 实时定量PCR 食源性致病菌 沙门氏菌 |
Establishment and Evaluation of Real-time PCR for Food-borne Salmonella Detection |
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| ZHENG Ming,ZHENG Bao-liang.Establishment and Evaluation of Real-time PCR for Food-borne Salmonella Detection[J].Journal of Zhengzhou College of Animal Husbandry Engineering,2010,30(1). |
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| Authors: | ZHENG Ming ZHENG Bao-liang |
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| Institution: | Biology department of Zhengzhou College of Animal Husbandry Engineering/a>;Henen Zhengzhou 450011/a>;China |
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| Abstract: | According to the inv A gene(AE008832) of Salmonella from GenBank,a pair of primers were designed to establish a real-time quantitative PCR assay.The correlation coefficient of standard curve was 0.999 and The minimal detection limit was 8 bacteria/reaction.Detecting 40 milk samples and 40 meat samples by real-time quantitative PCR,the positive rate was 2.5% and 5% respectively and the results was identical with the results by conventional microbiology method.Thus,the real-time quantitative PCR is more rapid... |
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| Keywords: | Real-time quantitative PCR Food-borne pathogenic bacteria Salmonella |
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